Infrapatellar fat pad adipose-derived stem cells co-cultured with articular chondrocytes from osteoarthritis patients exhibit increased chondrogenic gene expression.
Publication Date
2022-02-12Journal Title
Cell Commun Signal
ISSN
1478-811X
Publisher
Springer Science and Business Media LLC
Volume
20
Issue
1
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Mak, C. C., To, K., Fekir, K., Brooks, R., & Khan, W. (2022). Infrapatellar fat pad adipose-derived stem cells co-cultured with articular chondrocytes from osteoarthritis patients exhibit increased chondrogenic gene expression.. Cell Commun Signal, 20 (1) https://doi.org/10.1186/s12964-021-00815-x
Abstract
AIM: The variable results in clinical trials of adipose tissue-derived stem cells (ASCs) for chondral defects may be due to the different ex vivo culture conditions of the ASCs which are implanted to treat the lesions. We sought to determine the optimal in vitro chondrocyte co-culture condition that promotes infrapatellar fat pad-derived (IFPD) ASC chondrogenic gene expression in a novel co-culture combination. METHODS: In our study, we utilized an in vitro autologous co-culture of IFPD ASCs and articular chondrocytes derived from Kellgren-Lawrence Grade III/IV osteoarthritic human knee joints at ASC-to-chondrocyte seeding log ratios of 1:1, 10:1, and 100:1. Gene expression following in vitro co-culture was quantified by RT-qPCR with a panel comprising COL1A1, COL2A1, COL10A1, L-SOX5, SOX6, SOX9, ACAN, HSPG2, and COMP for chondrogenic gene expression. RESULTS: The chondrogenic gene expression profiles from co-cultures were greater than would be expected from an expression profile modeled from chondrocyte and ASC-only monocultures. Additionally, chondrogenic gene expression decreased with increasing ASC-to-chondrocyte seeding ratios. CONCLUSIONS: These findings provide insight into the mechanisms underlying clinical ASC therapies and signifies that IFPD ASCs pre-conditioned by chondrocyte co-culture may have improved chondrogenic potential for cartilage repair. This model can help further understand IFPD ASCs in chondral and osteochondral repair and the chondrogenic pathways involved. Video Abstract.
Keywords
Short Report, Mesenchymal stromal cells, Co-culture, Chondrogenesis, Cartilage, Cell-based therapy, Regenerative medicine, Osteoarthritis
Sponsorship
ARTHRITIS RESEARCH UK (21156)
Identifiers
s12964-021-00815-x, 815
External DOI: https://doi.org/10.1186/s12964-021-00815-x
This record's URL: https://www.repository.cam.ac.uk/handle/1810/333963
Rights
Licence:
http://creativecommons.org/licenses/by/4.0/
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