Maternal diet-induced obesity during pregnancy alters lipid supply to mouse E18.5 fetuses and changes the cardiac tissue lipidome in a sex- dependent manner
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Authors
Pantaleão, Lucas C
Inzani, Isabella
Furse, Samuel
Loche, Elena
Hufnagel, Antonia
Ashmore, Thomas
Blackmore, Heather L
Jenkins, Benjamin
Carpenter, Asha AM
Wilczynska, Ania
Bushell, Martin
Fernandez-Twinn, Denise S
Ozanne, Susan E
Publication Date
2022-01-13Journal Title
eLife
ISSN
2050-084X
Publisher
eLife Sciences Publications Ltd
Volume
11
Language
eng
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Pantaleão, L. C., Inzani, I., Furse, S., Loche, E., Hufnagel, A., Ashmore, T., Blackmore, H. L., et al. (2022). Maternal diet-induced obesity during pregnancy alters lipid supply to mouse E18.5 fetuses and changes the cardiac tissue lipidome in a sex- dependent manner. eLife, 11 https://doi.org/10.7554/eLife.69078
Abstract
Maternal obesity during pregnancy has immediate and long-term detrimental effects on the offspring heart. In this study, we characterized the cardiac and circulatory lipid profiles in late gestation E18.5 fetuses of diet-induced obese pregnant mice and established the changes in lipid abundance and fetal cardiac transcriptomics. We used untargeted and targeted lipidomics and transcriptomics to define changes in the serum and cardiac lipid composition and fatty acid metabolism in male and female fetuses. From these analyses we observed: (1) maternal obesity affects the maternal and fetal serum lipidome distinctly; (2) female fetal heart lipidomes are more sensitive to maternal obesity than males; (3) changes in lipid supply might contribute to early expression of lipolytic genes in mouse hearts exposed to maternal obesity. These results highlight the existence of sexually dimorphic responses of the fetal heart to the same in utero obesogenic environment and identify lipids species that might mediate programming of cardiovascular health.
Keywords
Biochemistry, Mouse, Fetal heart, developmental biology, Lipidomics, Maternal Obesity, Transcriptomics, Chemical Biology, Heart Metabolism
Sponsorship
MRC Metabolic Diseases Unit [MRC_MC_UU_00014/4]
Cambridge Home and EU Student Scholarship
British Heart Foundation studentship [FS/12/64/30001]
II was supported by a British Heart Foundation studentship [FS/18/56/35177]
Funder references
British Heart Foundation (RG/17/12/33167)
Wellcome Trust (208363/Z/17/Z)
Biotechnology and Biological Sciences Research Council (BB/M027252/1)
MRC (MC_UU_00014/4)
Medical Research Council (MC_UU_12012/4)
Biotechnology and Biological Sciences Research Council (BB/M027252/2)
Biotechnology and Biological Sciences Research Council (BB/M001865/1)
Identifiers
PMC8794468, 35025731
External DOI: https://doi.org/10.7554/eLife.69078
This record's URL: https://www.repository.cam.ac.uk/handle/1810/333984
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