Flip-Flap: A Simple Dual-View Imaging Method for 3D Reconstruction of Thick Plant Samples
Publication Date
2022-02-13Journal Title
Plants
Publisher
MDPI
Volume
11
Issue
4
Language
en
Type
Other
This Version
VoR
Metadata
Show full item recordCitation
Serra, L., Tan, S., Robinson, S., & Langdale, J. A. (2022). Flip-Flap: A Simple Dual-View Imaging Method for 3D Reconstruction of Thick Plant Samples. [Other]. https://doi.org/10.3390/plants11040506
Abstract
Plant development is a complex process that relies on molecular and cellular events being co-ordinated in space and time. Microscopy is one of the most powerful tools available to investigate this spatiotemporal complexity. One step towards a better understanding of complexity in plants would be the acquisition of 3D images of entire organs. However, 3D imaging of intact plant samples is not always simple and often requires expensive and/or non-trivial approaches. In particular, the inner tissues of thick samples are challenging to image. Here, we present the Flip-Flap method, a simple imaging protocol to produce 3D images of cleared plant samples at the organ scale. This method allows full 3D reconstruction of plant organs suitable for 3D segmentation and further related analysis and can be easily handled by relatively inexperienced microscopists.
Keywords
plant 3D imaging, multiview imaging, rice, barley, Marchantia
Sponsorship
Bill & (INV-002970)
Gatsby Charitable Foundation (GAT3395/CDE)
Royal Society (URF\R1\180196)
Identifiers
External DOI: https://doi.org/10.3390/plants11040506
This record's DOI: https://doi.org/10.17863/CAM.81455
Rights
Licence:
https://creativecommons.org/licenses/by/4.0/
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