Rapid and Modular DNA Assembly for Transformation of Marchantia Chloroplasts.

Authors
Frangedakis, Eftychios  ORCID logo  https://orcid.org/0000-0002-3483-8464
Markel, Kasey 
Sauret-Gueto, Susana 

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Type
Book chapter
Change log
Abstract

The bryophyte Marchantia polymorpha , has attracted significant attention as a powerful experimental system for studying aspects of plant biology including synthetic biology applications. We describe an efficient and simple recursive Type IIS DNA assembly method for the generation of DNA constructs for chloroplast genome manipulation, and an optimized technique for Marchantia chloroplast genome transformation. The utility of the system was demonstrated by the expression of a chloroplast codon-optimized cyan fluorescent protein.

Date
2021-06-07
Keywords
Chloroplast, DNA loop assembly, Marchantia, Transformation, Type IIS, Chloroplasts, DNA, Plant, Genetic Engineering, Marchantia, Plants, Genetically Modified, Synthetic Biology, Transformation, Genetic
Publisher
Humana
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/L014130/1)