Rapid and Modular DNA Assembly for Transformation of Marchantia Chloroplasts.

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Authors
  Frangedakis, Eftychios
Markel, Kasey
Sauret-Gueto, Susana
  Haseloff, Jim
Editors
Maliga, Pal
Publication Date
2021-06-07
Journal Title
Methods Mol Biol
ISSN
1064-3745
ISBN
978-1-0716-1472-3
Publisher
Humana
Volume
2317
Pages
343-365
Type
Book chapter
Physical Medium
Print
Metadata
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Citation
Frangedakis, E., Markel, K., Sauret-Gueto, S., & Haseloff, J. (2021). Rapid and Modular DNA Assembly for Transformation of Marchantia Chloroplasts.. In Maliga, Pal. Humana, Methods Mol Biol. [Book chapter]. https://doi.org/10.1007/978-1-0716-1472-3_21
Abstract
The bryophyte Marchantia polymorpha , has attracted significant attention as a powerful experimental system for studying aspects of plant biology including synthetic biology applications. We describe an efficient and simple recursive Type IIS DNA assembly method for the generation of DNA constructs for chloroplast genome manipulation, and an optimized technique for Marchantia chloroplast genome transformation. The utility of the system was demonstrated by the expression of a chloroplast codon-optimized cyan fluorescent protein.
Keywords
Chloroplast, DNA loop assembly, Marchantia, Transformation, Type IIS, Chloroplasts, DNA, Plant, Genetic Engineering, Marchantia, Plants, Genetically Modified, Synthetic Biology, Transformation, Genetic
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/L014130/1)
Embargo Lift Date
2022-05-25
Identifiers
External DOI: https://doi.org/10.1007/978-1-0716-1472-3_21
This record's DOI: https://doi.org/10.17863/CAM.81608
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