Rapid and Modular DNA Assembly for Transformation of Marchantia Chloroplasts.

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dc.contributor.authorFrangedakis, Eftychios
dc.contributor.authorMarkel, Kasey
dc.contributor.authorSauret-Gueto, Susana
dc.contributor.authorHaseloff, Jim
dc.contributor.editorMaliga, Pal
dc.date.accessioned2022-02-18T11:12:50Z
dc.date.available2022-02-18T11:12:50Z
dc.date.issued2021-06-07
dc.identifier.isbn978-1-0716-1472-3
dc.identifier.issn1064-3745
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/334196
dc.description.abstractThe bryophyte Marchantia polymorpha , has attracted significant attention as a powerful experimental system for studying aspects of plant biology including synthetic biology applications. We describe an efficient and simple recursive Type IIS DNA assembly method for the generation of DNA constructs for chloroplast genome manipulation, and an optimized technique for Marchantia chloroplast genome transformation. The utility of the system was demonstrated by the expression of a chloroplast codon-optimized cyan fluorescent protein.
dc.format.mediumPrint
dc.publisherHumana
dc.subjectChloroplast
dc.subjectDNA loop assembly
dc.subjectMarchantia
dc.subjectTransformation
dc.subjectType IIS
dc.subjectChloroplasts
dc.subjectDNA, Plant
dc.subjectGenetic Engineering
dc.subjectMarchantia
dc.subjectPlants, Genetically Modified
dc.subjectSynthetic Biology
dc.subjectTransformation, Genetic
dc.titleRapid and Modular DNA Assembly for Transformation of Marchantia Chloroplasts.
dc.typeBook chapter
dc.publisher.departmentDepartment of Plant Sciences
dc.date.updated2022-02-09T15:18:47Z
prism.endingPage365
prism.publicationDate2021
prism.publicationNameMethods Mol Biol
prism.startingPage343
prism.volume2317
dc.identifier.doi10.17863/CAM.81608
dcterms.dateAccepted2021-06-07
rioxxterms.versionofrecord10.1007/978-1-0716-1472-3_21
dc.contributor.orcidFrangedakis, Eftychios [0000-0002-3483-8464]
dc.contributor.orcidHaseloff, Jim [0000-0003-4793-8058]
dc.identifier.eissn1940-6029
dcterms.isPartOfChloroplast Biotechnology: Methods and Protocols
rioxxterms.typeBook chapter
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/L014130/1)
cam.issuedOnline2021-05-25
cam.depositDate2022-02-09
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement
rioxxterms.freetoread.startdate2022-05-25


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