Rapid and Modular DNA Assembly for Transformation of Marchantia Chloroplasts.
Accepted version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Frangedakis, Eftychios https://orcid.org/0000-0002-3483-8464
Markel, Kasey
Sauret-Gueto, Susana
Haseloff, Jim https://orcid.org/0000-0003-4793-8058
Abstract
The bryophyte Marchantia polymorpha , has attracted significant attention as a powerful experimental system for studying aspects of plant biology including synthetic biology applications. We describe an efficient and simple recursive Type IIS DNA assembly method for the generation of DNA constructs for chloroplast genome manipulation, and an optimized technique for Marchantia chloroplast genome transformation. The utility of the system was demonstrated by the expression of a chloroplast codon-optimized cyan fluorescent protein.
Description
Title
Rapid and Modular DNA Assembly for Transformation of Marchantia Chloroplasts.
Keywords
Chloroplast, DNA loop assembly, Marchantia, Transformation, Type IIS, Chloroplasts, DNA, Plant, Genetic Engineering, Marchantia, Plants, Genetically Modified, Synthetic Biology, Transformation, Genetic
Is Part Of
Chloroplast Biotechnology: Methods and Protocols
Book type
Publisher
Humana
Publisher DOI
ISBN
978-1-0716-1472-3
Rights
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/L014130/1)