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dc.contributor.authorPalmer, Phil
dc.contributor.authorDel Rosario, Joanne Marie M
dc.contributor.authorda Costa, Kelly AS
dc.contributor.authorCarnell, George W
dc.contributor.authorHuang, Chloe Q
dc.contributor.authorHeeney, Jonathan L
dc.contributor.authorTemperton, Nigel J
dc.contributor.authorWells, David A
dc.date.accessioned2022-02-24T09:00:59Z
dc.date.available2022-02-24T09:00:59Z
dc.date.issued2022-02-10
dc.date.submitted2021-03-16
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/334403
dc.description.abstract<jats:p>The emergence of COVID-19 has emphasised that biological assay data must be analysed quickly to develop safe, effective and timely vaccines/therapeutics. For viruses such as SARS-CoV-2, the primary way of measuring immune correlates of protection is through assays such as the pseudotype microneutralisation (pMN) assay, thanks to its safety and versatility. However, despite the presence of existing tools for data analysis such as PRISM and R the analysis of these assays remains cumbersome and time-consuming. We introduce an open-source R Shiny web application and R library (AutoPlate) to accelerate data analysis of dose-response curve immunoassays. Using example data from influenza studies, we show that AutoPlate improves on available analysis software in terms of ease of use, flexibility and speed. AutoPlate (<jats:uri>https://philpalmer.shinyapps.io/AutoPlate/</jats:uri>) is a tool for the use of laboratories and wider scientific community to accelerate the analysis of biological assays in the development of viral vaccines and therapeutics.</jats:p>
dc.languageen
dc.publisherFrontiers Media SA
dc.subjectImmunology
dc.subjectpseudotype neutralization
dc.subjectELLA
dc.subjectdata analysis
dc.subjectbiological assay
dc.subjectdose-response
dc.subjectpseudovirus
dc.subjectSARS-C0V-2
dc.subjectinfluenza
dc.titleAutoPlate: Rapid Dose-Response Curve Analysis for Biological Assays
dc.typeArticle
dc.date.updated2022-02-24T09:00:59Z
prism.publicationNameFrontiers in Immunology
prism.volume12
dc.identifier.doi10.17863/CAM.81819
dcterms.dateAccepted2021-12-29
rioxxterms.versionofrecord10.3389/fimmu.2021.681636
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidCarnell, George [0000-0001-8875-0989]
dc.contributor.orcidHeeney, Jonathan [0000-0003-2702-1621]
dc.identifier.eissn1664-3224
cam.issuedOnline2022-02-10


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