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dc.contributor.authorSpitz, Olivia
dc.contributor.authorErenburg, Isabelle N
dc.contributor.authorKanonenberg, Kerstin
dc.contributor.authorPeherstorfer, Sandra
dc.contributor.authorLenders, Michael HH
dc.contributor.authorReiners, Jens
dc.contributor.authorMa, Miao
dc.contributor.authorLuisi, Ben F
dc.contributor.authorSmits, Sander HJ
dc.contributor.authorSchmitt, Lutz
dc.date.accessioned2022-02-24T20:00:17Z
dc.date.available2022-02-24T20:00:17Z
dc.date.issued2022-02-10
dc.date.submitted2021-10-29
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/334436
dc.description.abstract<jats:p>The toxin hemolysin A was first identified in uropathogenic <jats:italic>E. coli</jats:italic> strains and shown to be secreted in a one-step mechanism by a dedicated secretion machinery. This machinery, which belongs to the Type I secretion system family of the Gram-negative bacteria, is composed of the outer membrane protein TolC, the membrane fusion protein HlyD and the ABC transporter HlyB. The N-terminal domain of HlyA represents the toxin which is followed by a RTX (Repeats in Toxins) domain harboring nonapeptide repeat sequences and the secretion signal at the extreme C-terminus. This secretion signal, which is necessary and sufficient for secretion, does not appear to require a defined sequence, and the nature of the encoded signal remains unknown. Here, we have combined structure prediction based on the AlphaFold algorithm together with functional and <jats:italic>in silico</jats:italic> data to examine the role of secondary structure in secretion. Based on the presented data, a C-terminal, amphipathic helix is proposed between residues 975 and 987 that plays an essential role in the early steps of the secretion process.</jats:p>
dc.languageen
dc.publisherFrontiers Media SA
dc.subjectPhysiology
dc.subjectbacterial secretion systems
dc.subjectsecretion signal
dc.subjectABC transporter
dc.subjectamphipathic helix
dc.subjectATPase activity
dc.subjectprotein secretion
dc.titleIdentity Determinants of the Translocation Signal for a Type 1 Secretion System
dc.typeArticle
dc.date.updated2022-02-24T20:00:16Z
prism.publicationNameFrontiers in Physiology
prism.volume12
dc.identifier.doi10.17863/CAM.81851
dcterms.dateAccepted2021-12-31
rioxxterms.versionofrecord10.3389/fphys.2021.804646
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidLuisi, Ben [0000-0003-1144-9877]
dc.identifier.eissn1664-042X
cam.issuedOnline2022-02-10


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