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dc.contributor.authorKuchipudi, Suresh V
dc.contributor.authorSurendran-Nair, Meera
dc.contributor.authorRuden, Rachel M
dc.contributor.authorYon, Michele
dc.contributor.authorNissly, Ruth H
dc.contributor.authorVandegrift, Kurt J
dc.contributor.authorNelli, Rahul K
dc.contributor.authorLi, Lingling
dc.contributor.authorJayarao, Bhushan M
dc.contributor.authorMaranas, Costas D
dc.contributor.authorLevine, Nicole
dc.contributor.authorWillgert, Katriina
dc.contributor.authorConlan, Andrew
dc.contributor.authorOlsen, Randall J
dc.contributor.authorDavis, James J
dc.contributor.authorMusser, James M
dc.contributor.authorHudson, Peter J
dc.contributor.authorKapur, Vivek
dc.date.accessioned2022-02-27T02:02:23Z
dc.date.available2022-02-27T02:02:23Z
dc.date.issued2022-02-08
dc.identifier.issn0027-8424
dc.identifier.otherPMC8833191
dc.identifier.other35078920
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/334480
dc.description.abstractMany animal species are susceptible to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and could act as reservoirs; however, transmission in free-living animals has not been documented. White-tailed deer, the predominant cervid in North America, are susceptible to SARS-CoV-2 infection, and experimentally infected fawns can transmit the virus. To test the hypothesis that SARS-CoV-2 is circulating in deer, 283 retropharyngeal lymph node (RPLN) samples collected from 151 free-living and 132 captive deer in Iowa from April 2020 through January of 2021 were assayed for the presence of SARS-CoV-2 RNA. Ninety-four of the 283 (33.2%) deer samples were positive for SARS-CoV-2 RNA as assessed by RT-PCR. Notably, following the November 2020 peak of human cases in Iowa, and coinciding with the onset of winter and the peak deer hunting season, SARS-CoV-2 RNA was detected in 80 of 97 (82.5%) RPLN samples collected over a 7-wk period. Whole genome sequencing of all 94 positive RPLN samples identified 12 SARS-CoV-2 lineages, with B.1.2 (n = 51; 54.5%) and B.1.311 (n = 19; 20%) accounting for ∼75% of all samples. The geographic distribution and nesting of clusters of deer and human lineages strongly suggest multiple human-to-deer transmission events followed by subsequent deer-to-deer spread. These discoveries have important implications for the long-term persistence of the SARS-CoV-2 pandemic. Our findings highlight an urgent need for a robust and proactive "One Health" approach to obtain enhanced understanding of the ecology, molecular evolution, and dissemination of SARS-CoV-2.
dc.languageeng
dc.publisherProceedings of the National Academy of Sciences
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcenlmid: 7505876
dc.sourceessn: 1091-6490
dc.subjectSpillover
dc.subjectDeer
dc.subjectAnimal Reservoir
dc.subjectOne Health
dc.subjectSars-cov-2
dc.subjectAnimals
dc.subjectHumans
dc.subjectZoonoses
dc.subjectDisease Reservoirs
dc.subjectCOVID-19
dc.subjectSARS-CoV-2
dc.titleMultiple spillovers from humans and onward transmission of SARS-CoV-2 in white-tailed deer.
dc.typeArticle
dc.date.updated2022-02-27T02:02:22Z
prism.issueIdentifier6
prism.publicationNameProc Natl Acad Sci U S A
prism.volume119
dc.identifier.doi10.17863/CAM.81897
dcterms.dateAccepted2021-12-16
rioxxterms.versionofrecord10.1073/pnas.2121644119
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidSurendran-Nair, Meera [0000-0003-4686-8414]
dc.contributor.orcidRuden, Rachel M [0000-0003-3764-9447]
dc.contributor.orcidNissly, Ruth H [0000-0002-3102-7447]
dc.contributor.orcidNelli, Rahul K [0000-0001-8765-0943]
dc.contributor.orcidWillgert, Katriina [0000-0003-3586-1997]
dc.contributor.orcidConlan, Andrew [0000-0002-2593-6353]
dc.contributor.orcidMusser, James M [0000-0002-7765-4956]
dc.contributor.orcidHudson, Peter J [0000-0003-0468-3403]
dc.contributor.orcidKapur, Vivek [0000-0002-9648-0138]
dc.identifier.eissn1091-6490
pubs.funder-project-idNIAID NIH HHS (75N93019C00076)
cam.issuedOnline2022-01-25


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International