Single-cell transcriptomics links malignant T cells to the tumor immune landscape in cutaneous T cell lymphoma.
Authors
Liu, Xiangjun
Hu, Simeng
Li, Ruoyan
Pan, Haihao
Lai, Pan
Xu, Deshu
Sun, Jingru
Gao, Yumei
Liu, Fengjie
Xiao, Yu
Li, Yingyi
Wen, Yujie
Chen, Zhuojing
Xu, Bufang
Lin, Yuchieh
Ran, Menglong
Li, Qianxi
Yang, Shuxia
Li, Hang
Tu, Ping
Publication Date
2022-03-03Journal Title
Nat Commun
ISSN
2041-1723
Publisher
Springer Science and Business Media LLC
Volume
13
Issue
1
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Liu, X., Jin, S., Hu, S., Li, R., Pan, H., Liu, Y., Lai, P., et al. (2022). Single-cell transcriptomics links malignant T cells to the tumor immune landscape in cutaneous T cell lymphoma.. Nat Commun, 13 (1) https://doi.org/10.1038/s41467-022-28799-3
Description
Funder: National Nature Science Foundation of China 81922058 National Science and Technology Major Project 2019YFC1315702 Guangdong Province Key Research and Development Program 2019B020226002 National Youth Top-Notch Talent Support Program 283812 Peking University Clinical Medicine plus X Youth Project PKU2019LCXQ012
Abstract
Cutaneous T cell lymphoma (CTCL) represents a heterogeneous group of non-Hodgkin lymphoma distinguished by the presence of clonal malignant T cells. The heterogeneity of malignant T cells and the complex tumor microenvironment remain poorly characterized. With single-cell RNA analysis and bulk whole-exome sequencing on 19 skin lesions from 15 CTCL patients, we decipher the intra-tumor and inter-lesion diversity of CTCL patients and propose a multi-step tumor evolution model. We further establish a subtyping scheme based on the molecular features of malignant T cells and their pro-tumorigenic microenvironments: the TCyEM group, demonstrating a cytotoxic effector memory T cell phenotype, shows more M2 macrophages infiltration, while the TCM group, featured by a central memory T cell phenotype and adverse patient outcome, is infiltrated by highly exhausted CD8+ reactive T cells, B cells and Tregs with suppressive activities. Our results establish a solid basis for understanding the nature of CTCL and pave the way for future precision medicine for CTCL patients.
Keywords
Article, /631/250/580, /692/4028/67/1813, /692/4028/67/2329, /692/420/755, /631/67/1990/291/1621/1916, /49/91, article
Identifiers
s41467-022-28799-3, 28799
External DOI: https://doi.org/10.1038/s41467-022-28799-3
This record's URL: https://www.repository.cam.ac.uk/handle/1810/334643
Rights
Licence:
http://creativecommons.org/licenses/by/4.0/
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