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dc.contributor.authorSanchez-Burgos, Ignacio
dc.contributor.authorEspinosa, Jorge R
dc.contributor.authorJoseph, Jerelle
dc.contributor.authorCollepardo-Guevara, Rosana
dc.date.accessioned2022-03-04T21:00:19Z
dc.date.available2022-03-04T21:00:19Z
dc.date.issued2022-02
dc.date.submitted2021-10-12
dc.identifier.issn1553-734X
dc.identifier.otherpcompbiol-d-21-01836
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/334677
dc.descriptionFunder: Oppenheimer Fellowship
dc.descriptionFunder: Roger Ekins Fellowship
dc.descriptionFunder: Derek Brewer Emmanuel College scholarship
dc.description.abstractBiomolecular condensates formed via liquid-liquid phase separation (LLPS) play a crucial role in the spatiotemporal organization of the cell material. Nucleic acids can act as critical modulators in the stability of these protein condensates. To unveil the role of RNA length in regulating the stability of RNA binding protein (RBP) condensates, we present a multiscale computational strategy that exploits the advantages of a sequence-dependent coarse-grained representation of proteins and a minimal coarse-grained model wherein proteins are described as patchy colloids. We find that for a constant nucleotide/protein ratio, the protein fused in sarcoma (FUS), which can phase separate on its own-i.e., via homotypic interactions-only exhibits a mild dependency on the RNA strand length. In contrast, the 25-repeat proline-arginine peptide (PR25), which does not undergo LLPS on its own at physiological conditions but instead exhibits complex coacervation with RNA-i.e., via heterotypic interactions-shows a strong dependence on the length of the RNA strands. Our minimal patchy particle simulations suggest that the strikingly different effect of RNA length on homotypic LLPS versus RBP-RNA complex coacervation is general. Phase separation is RNA-length dependent whenever the relative contribution of heterotypic interactions sustaining LLPS is comparable or higher than those stemming from protein homotypic interactions. Taken together, our results contribute to illuminate the intricate physicochemical mechanisms that influence the stability of RBP condensates through RNA inclusion.
dc.languageen
dc.publisherPublic Library of Science (PLoS)
dc.subjectResearch Article
dc.subjectBiology and life sciences
dc.subjectComputer and information sciences
dc.subjectPhysical sciences
dc.titleRNA length has a non-trivial effect in the stability of biomolecular condensates formed by RNA-binding proteins.
dc.typeArticle
dc.date.updated2022-03-04T21:00:18Z
prism.issueIdentifier2
prism.publicationNamePLoS Comput Biol
prism.volume18
dc.identifier.doi10.17863/CAM.82095
dcterms.dateAccepted2022-01-06
rioxxterms.versionofrecord10.1371/journal.pcbi.1009810
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
datacite.contributor.supervisoreditor: Chen, Shi-Jie
dc.contributor.orcidSanchez-Burgos, Ignacio [0000-0002-1160-3945]
dc.contributor.orcidEspinosa, Jorge R [0000-0001-9530-2658]
dc.contributor.orcidJoseph, Jerelle [0000-0003-4525-180X]
dc.contributor.orcidCollepardo-Guevara, Rosana [0000-0003-1781-7351]
dc.identifier.eissn1553-7358
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/P020259/1)
pubs.funder-project-idEuropean Research Council (803326)
pubs.funder-project-idEPSRC (EP/T517847/1)
cam.issuedOnline2022-02-02


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