Lima1 mediates the pluripotency control of membrane dynamics and cellular metabolism.
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Authors
Duethorn, Binyamin
Groll, Fabian
Rieger, Bettina
Brinkmann, Heike
Kremer, Ludmila
Stehling, Martin
Mildner, Karina
Busch, Karin B
Publication Date
2022-02-01Journal Title
Nat Commun
ISSN
2041-1723
Publisher
Springer Science and Business Media LLC
Volume
13
Issue
1
Language
eng
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Duethorn, B., Groll, F., Rieger, B., Drexler, H. C., Brinkmann, H., Kremer, L., Stehling, M., et al. (2022). Lima1 mediates the pluripotency control of membrane dynamics and cellular metabolism.. Nat Commun, 13 (1) https://doi.org/10.1038/s41467-022-28139-5
Abstract
Lima1 is an extensively studied prognostic marker of malignancy and is also considered to be a tumour suppressor, but its role in a developmental context of non-transformed cells is poorly understood. Here, we characterise the expression pattern and examined the function of Lima1 in mouse embryos and pluripotent stem cell lines. We identify that Lima1 expression is controlled by the naïve pluripotency circuit and is required for the suppression of membrane blebbing, as well as for proper mitochondrial energetics in embryonic stem cells. Moreover, forcing Lima1 expression enables primed mouse and human pluripotent stem cells to be incorporated into murine pre-implantation embryos. Thus, Lima1 is a key effector molecule that mediates the pluripotency control of membrane dynamics and cellular metabolism.
Keywords
Pluripotent Stem Cells, Blastocyst, Animals, Mice, Cytoskeletal Proteins, Cell Proliferation, Embryonic Development, Female, Male, Embryonic Stem Cells
Sponsorship
Wellcome Trust (098287/Z/12/Z)
European Research Council (669198)
Identifiers
PMC8807836, 35105859
External DOI: https://doi.org/10.1038/s41467-022-28139-5
This record's URL: https://www.repository.cam.ac.uk/handle/1810/334709
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