NuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis.
View / Open Files
Authors
Lachance, Catherine
Kabza, Michał
De Winne, Nancy
De Jaeger, Geert
Sadowski, Jan
Publication Date
2022-01-12Journal Title
Nat Commun
ISSN
2041-1723
Publisher
Springer Science and Business Media LLC
Volume
13
Issue
1
Number
ARTN 277
Pages
277
Type
Article
This Version
VoR
Physical Medium
Electronic
Metadata
Show full item recordCitation
Bieluszewski, T., Sura, W., Dziegielewski, W., Bieluszewska, A., Lachance, C., Kabza, M., Szymanska-Lejman, M., et al. (2022). NuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis.. Nat Commun, 13 (1. ARTN 277), 277. https://doi.org/10.1038/s41467-021-27882-5
Abstract
Nucleosomal acetyltransferase of H4 (NuA4) is an essential transcriptional coactivator in eukaryotes, but remains poorly characterized in plants. Here, we describe Arabidopsis homologs of the NuA4 scaffold proteins Enhancer of Polycomb-Like 1 (AtEPL1) and Esa1-Associated Factor 1 (AtEAF1). Loss of AtEAF1 results in inhibition of growth and chloroplast development. These effects are stronger in the Atepl1 mutant and are further enhanced by loss of Golden2-Like (GLK) transcription factors, suggesting that NuA4 activates nuclear plastid genes alongside GLK. We demonstrate that AtEPL1 is necessary for nucleosomal acetylation of histones H4 and H2A.Z by NuA4 in vitro. These chromatin marks are diminished genome-wide in Atepl1, while another active chromatin mark, H3K9 acetylation (H3K9ac), is locally enhanced. Expression of many chloroplast-related genes depends on NuA4, as they are downregulated with loss of H4ac and H2A.Zac. Finally, we demonstrate that NuA4 promotes H2A.Z deposition and by doing so prevents spurious activation of stress response genes.
Keywords
Acetyltransferases, Arabidopsis, Arabidopsis Proteins, Autotrophic Processes, Cell Nucleus, Chloroplasts, Chromatin, Ephrin-A1, Gene Expression Regulation, Plant, Histones, Nuclear Pore Complex Proteins, Nucleosomes, Stress, Physiological, Transcription Factors
Identifiers
External DOI: https://doi.org/10.1038/s41467-021-27882-5
This record's URL: https://www.repository.cam.ac.uk/handle/1810/334874
Statistics
Total file downloads (since January 2020). For more information on metrics see the
IRUS guide.
Recommended or similar items
The current recommendation prototype on the Apollo Repository will be turned off on 03 February 2023. Although the pilot has been fruitful for both parties, the service provider IKVA is focusing on horizon scanning products and so the recommender service can no longer be supported. We recognise the importance of recommender services in supporting research discovery and are evaluating offerings from other service providers. If you would like to offer feedback on this decision please contact us on: support@repository.cam.ac.uk