Multiple phylogenetically-diverse, differentially-virulent Burkholderia pseudomallei isolated from a single soil sample collected in Thailand.
View / Open Files
Authors
Vazquez, Adam J
Phillips, Paul D
Allender, Chris J
Bowen, Richard A
Nottingham, Roxanne D
Doyle, Adina
Wongsuwan, Gumphol
Wuthiekanun, Vanaporn
Keim, Paul
Publication Date
2022-02Journal Title
PLoS Negl Trop Dis
ISSN
1935-2727
Publisher
Public Library of Science (PLoS)
Volume
16
Issue
2
Language
eng
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Roe, C., Vazquez, A. J., Phillips, P. D., Allender, C. J., Bowen, R. A., Nottingham, R. D., Doyle, A., et al. (2022). Multiple phylogenetically-diverse, differentially-virulent Burkholderia pseudomallei isolated from a single soil sample collected in Thailand.. PLoS Negl Trop Dis, 16 (2) https://doi.org/10.1371/journal.pntd.0010172
Abstract
Burkholderia pseudomallei is a soil-dwelling bacterium endemic to Southeast Asia and northern Australia that causes the disease, melioidosis. Although the global genomic diversity of clinical B. pseudomallei isolates has been investigated, there is limited understanding of its genomic diversity across small geographic scales, especially in soil. In this study, we obtained 288 B. pseudomallei isolates from a single soil sample (~100g; intensive site 2, INT2) collected at a depth of 30cm from a site in Ubon Ratchathani Province, Thailand. We sequenced the genomes of 169 of these isolates that represent 7 distinct sequence types (STs), including a new ST (ST1820), based on multi-locus sequence typing (MLST) analysis. A core genome SNP phylogeny demonstrated that all identified STs share a recent common ancestor that diverged an estimated 796-1260 years ago. A pan-genomics analysis demonstrated recombination between clades and intra-MLST phylogenetic and gene differences. To identify potential differential virulence between STs, groups of BALB/c mice (5 mice/isolate) were challenged via subcutaneous injection (500 CFUs) with 30 INT2 isolates representing 5 different STs; over the 21-day experiment, eight isolates killed all mice, 2 isolates killed an intermediate number of mice (1-2), and 20 isolates killed no mice. Although the virulence results were largely stratified by ST, one virulent isolate and six attenuated isolates were from the same ST (ST1005), suggesting that variably conserved genomic regions may contribute to virulence. Genomes from the animal-challenged isolates were subjected to a bacterial genome-wide association study to identify genomic regions associated with differential virulence. One associated region is a unique variant of Hcp1, a component of the type VI secretion system, which may result in attenuation. The results of this study have implications for comprehensive sampling strategies, environmental exposure risk assessment, and understanding recombination and differential virulence in B. pseudomallei.
Keywords
Animals, Mice, Inbred BALB C, Humans, Burkholderia pseudomallei, Melioidosis, Genomics, Soil Microbiology, Phylogeny, Virulence, Genome, Bacterial, Thailand, Female, Multilocus Sequence Typing
Identifiers
PMC8865643, 35143500
External DOI: https://doi.org/10.1371/journal.pntd.0010172
This record's URL: https://www.repository.cam.ac.uk/handle/1810/334938
Statistics
Total file downloads (since January 2020). For more information on metrics see the
IRUS guide.
Recommended or similar items
The current recommendation prototype on the Apollo Repository will be turned off on 03 February 2023. Although the pilot has been fruitful for both parties, the service provider IKVA is focusing on horizon scanning products and so the recommender service can no longer be supported. We recognise the importance of recommender services in supporting research discovery and are evaluating offerings from other service providers. If you would like to offer feedback on this decision please contact us on: support@repository.cam.ac.uk