B cell receptor repertoire kinetics after SARS-CoV-2 infection and vaccination.
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Authors
Kotagiri, Prasanti
Mescia, Federica
Rae, William M
Bergamaschi, Laura
Tuong, Zewen K
Turner, Lorinda
Hunter, Kelvin
Gerber, Pehuén P
Cambridge Institute of Therapeutic Immunology and Infectious Disease-National Institute of Health Research (CITIID-NIHR) COVID BioResource Collaboration
Hess, Christoph
Clatworthy, Menna R
Goodfellow, Ian G
Matheson, Nicholas J
McKinney, Eoin F
Wills, Mark R
Gupta, Ravindra K
Bradley, John R
Bashford-Rogers, Rachael JM
Lyons, Paul A
Smith, Kenneth GC
Publication Date
2022-01-31Journal Title
Cell Reports
ISSN
2211-1247
Publisher
Elsevier
Volume
38
Issue
7
Language
eng
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Kotagiri, P., Mescia, F., Rae, W. M., Bergamaschi, L., Tuong, Z. K., Turner, L., Hunter, K., et al. (2022). B cell receptor repertoire kinetics after SARS-CoV-2 infection and vaccination.. Cell Reports, 38 (7) https://doi.org/10.1016/j.celrep.2022.110393
Description
Funder: Medical Research Council
Funder: National Institute for Health Research (NIHR)
Abstract
B cells are important in immunity to both severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection and vaccination, but B cell receptor (BCR) repertoire development in these contexts has not been compared. We analyze serial samples from 171 SARS-CoV-2-infected individuals and 63 vaccine recipients and find the global BCR repertoire differs between them. Following infection, immunoglobulin (Ig)G1/3 and IgA1 BCRs increase, somatic hypermutation (SHM) decreases, and, in severe disease, IgM and IgA clones are expanded. In contrast, after vaccination, the proportion of IgD/M BCRs increase, SHM is unchanged, and expansion of IgG clones is prominent. VH1-24, which targets the N-terminal domain (NTD) and contributes to neutralization, is expanded post infection except in the most severe disease. Infection generates a broad distribution of SARS-CoV-2-specific clones predicted to target the spike protein, while a more focused response after vaccination mainly targets the spike's receptor-binding domain. Thus, the nature of SARS-CoV-2 exposure differentially affects BCR repertoire development, potentially informing vaccine strategies.
Keywords
B Cell Receptor Repertoire, Covid-19, Sars-Cov-2 Vaccination, B-Lymphocytes, Humans, Immunoglobulin Variable Region, Receptors, Antigen, B-Cell, Immunoglobulin Isotypes, Vaccination, Severity of Illness Index, Somatic Hypermutation, Immunoglobulin, Kinetics, Immunoglobulin Heavy Chains, Clonal Evolution, Spike Glycoprotein, Coronavirus, COVID-19, SARS-CoV-2, BNT162 Vaccine
Sponsorship
Wellcome Trust (200871/Z/16/Z)
Medical Research Council (MR/P008801/1)
Wellcome Trust (207498/Z/17/Z)
Medical Research Council (MR/S035842/1)
MRC (via University of Birmingham) (MR/V028448/1)
Identifiers
PMC8801326, 35143756
External DOI: https://doi.org/10.1016/j.celrep.2022.110393
This record's URL: https://www.repository.cam.ac.uk/handle/1810/334942
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