Evaluation of Label-Free Confocal Raman Microspectroscopy for Monitoring Oxidative Stress In Vitro in Live Human Cancer Cells
Publication Date
2022-03-17Journal Title
Antioxidants
Publisher
MDPI AG
Volume
11
Issue
3
Language
en
Type
Article
This Version
VoR
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Surmacki, J. M., Quiros-Gonzalez, I., & Bohndiek, S. E. (2022). Evaluation of Label-Free Confocal Raman Microspectroscopy for Monitoring Oxidative Stress In Vitro in Live Human Cancer Cells. Antioxidants, 11 (3) https://doi.org/10.3390/antiox11030573
Abstract
<jats:p>Understanding the impact of free radicals and antioxidants in cell biology is vital; however, noninvasive nonperturbative imaging of oxidative stress remains a challenge. Here, we evaluated the ability of label-free Raman spectroscopy to monitor redox biochemical changes in antioxidant (N-acetyl-l-cysteine, NAC) and pro-oxidant (tert-butyl hydroperoxide, TBHP) environments. Cellular changes were compared to fluorescence microscopy using CellROX Orange as a marker of oxidative stress. We also investigated the influence of cell media with and without serum. Incubation of cells with NAC increased the Raman signal at 498 cm−1 from S-S disulphide stretching mode, one of the most important redox-related sensors. Exposure of cells to TBHP resulted in decreased Raman spectral signals from DNA/proteins and lipids (at 784, 1094, 1003, 1606, 1658 and 718, 1264, 1301, 1440, 1746 cm−1). Using partial least squares–discriminant analysis, we showed that Raman spectroscopy can achieve sensitivity up to 96.7%, 94.8% and 91.6% for control, NAC and TBHP conditions, respectively, with specificity of up to 93.5, 90.1% and 87.9%. Our results indicate that Raman spectroscopy can directly measure the effect of NAC antioxidants and accurately characterize the intracellular conditions associated with TBHP-induced oxidative stress, including lipid peroxidation and DNA damage.</jats:p>
Keywords
oxidative stress, Raman microspectroscopy, fluorescence microscopy, tert-butyl hydroperoxide, N-acetyl-l-cysteine
Sponsorship
Cancer Research UK (C14303/A17197, C47594/A16267)
European Union (FP7-PEOPLE-2013-CIG-630729)
Identifiers
External DOI: https://doi.org/10.3390/antiox11030573
This record's URL: https://www.repository.cam.ac.uk/handle/1810/335237
Rights
Licence:
https://creativecommons.org/licenses/by/4.0/
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