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dc.contributor.authorWheeler, Lucas C
dc.contributor.authorWalker, Joseph F
dc.contributor.authorNg, Julienne
dc.contributor.authorDeanna, Rocío
dc.contributor.authorDunbar-Wallis, Amy
dc.contributor.authorBackes, Alice
dc.contributor.authorPezzi, Pedro H
dc.contributor.authorPalchetti, M Virginia
dc.contributor.authorRobertson, Holly M
dc.contributor.authorMonaghan, Andrew
dc.contributor.authorde Freitas, Loreta Brandão
dc.contributor.authorBarboza, Gloria E
dc.contributor.authorMoyroud, Edwige
dc.contributor.authorSmith, Stacey D
dc.date.accessioned2022-03-28T19:07:18Z
dc.date.available2022-03-28T19:07:18Z
dc.date.issued2022-03-02
dc.identifier.issn0737-4038
dc.identifier.other35212724
dc.identifier.otherPMC8911815
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/335431
dc.description.abstractDissecting the relationship between gene function and substitution rates is key to understanding genome-wide patterns of molecular evolution. Biochemical pathways provide powerful systems for investigating this relationship because the functional role of each gene is often well characterized. Here, we investigate the evolution of the flavonoid pigment pathway in the colorful Petunieae clade of the tomato family (Solanaceae). This pathway is broadly conserved in plants, both in terms of its structural elements and its MYB, basic helix-loop-helix, and WD40 transcriptional regulators, and its function has been extensively studied, particularly in model species of petunia. We built a phylotranscriptomic data set for 69 species of Petunieae to infer patterns of molecular evolution across pathway genes and across lineages. We found that transcription factors exhibit faster rates of molecular evolution (dN/dS) than their targets, with the highly specialized MYB genes evolving fastest. Using the largest comparative data set to date, we recovered little support for the hypothesis that upstream enzymes evolve slower than those occupying more downstream positions, although expression levels do predict molecular evolutionary rates. Although shifts in floral pigmentation were only weakly related to changes affecting coding regions, we found a strong relationship with the presence/absence patterns of MYB transcripts. Intensely pigmented species express all three main MYB anthocyanin activators in petals, whereas pale or white species express few or none. Our findings reinforce the notion that pathway regulators have a dynamic history, involving higher rates of molecular evolution than structural components, along with frequent changes in expression during color transitions.
dc.description.sponsorshipGatsby Charitable Foundation Fellowship
dc.languageeng
dc.publisherOxford University Press (OUP)
dc.rightsAttribution-NonCommercial 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.sourceessn: 1537-1719
dc.sourcenlmid: 8501455
dc.subjectSolanaceae
dc.subjectMolecular evolution
dc.subjectAnthocyanins
dc.subjectTranscription factors
dc.subjectFlower color
dc.subjectPhylogenomics
dc.subjectMyb
dc.subjectPetunieae
dc.titleTranscription Factors Evolve Faster Than Their Structural Gene Targets in the Flavonoid Pigment Pathway.
dc.typeArticle
dc.date.updated2022-03-28T19:07:17Z
prism.issueIdentifier3
prism.publicationNameMol Biol Evol
prism.volume39
dc.identifier.doi10.17863/CAM.82860
dcterms.dateAccepted2022-02-22
rioxxterms.versionofrecord10.1093/molbev/msac044
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.contributor.orcidWheeler, Lucas C [0000-0002-9483-9792]
dc.contributor.orcidMoyroud, Edwige [0000-0001-7908-3205]
dc.contributor.orcidSmith, Stacey D [0000-0003-2010-4459]
dc.identifier.eissn1537-1719
cam.issuedOnline2022-02-25


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Attribution-NonCommercial 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial 4.0 International