Highly sensitive fluorescence-based mercury(II) DNA sensor enhanced by silver(I) activation
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Authors
Allen, Jasmina
Kamboj, Varun S
Corns, Warren T
Verde-Luján, Hernán
Valladares, Luis De Los Santos
Villanueva, Carlos
Jhoncon, Jorge H
Barnes, Crispin HW
Publication Date
2022-04Journal Title
Measurement: Sensors
ISSN
2665-9174
Publisher
Elsevier BV
Volume
20-21
Number
100368
Pages
100368-100368
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Idros, N., Stott, K., Allen, J., Kamboj, V. S., Corns, W. T., Verde-Luján, H., Valladares, L. D. L. S., et al. (2022). Highly sensitive fluorescence-based mercury(II) DNA sensor enhanced by silver(I) activation. Measurement: Sensors, 20-21 (100368), 100368-100368. https://doi.org/10.1016/j.measen.2022.100368
Abstract
Mercury exposure constitutes an acute risk to human health and the environment. Driven by the requirement to monitor trace-level mercury, we report a highly sensitive mercury(II) DNA sensor enhanced by silver(I) activation, followed by mercury(II)-specific oligonucleotides (MSO) molecular configuration switch and mercury(II)-modulated FRET. Activating the MSO strands with silver(I), mismatched cytosine–silver(I)–cytosine bridges induce individual MSO strands to fold readily in response to mercury(II) resulting in enhanced fluorescence signal. The structural switches were studied by 2D 1H–1H NOESY and TOCSY NMR spectroscopy, and 260 nm absorbance. The signal decreases with increasing mercury(II) concentration from 100 pM to 0.1 mM. The approach affords outstanding mercury(II) selectivity over other environmentally associated metals. Furthermore, the methodology was deployed for detection of mercury(II) in spiked pond waters with 97.9–100.6% recovery. The simple and feasible format has great potential for developing a cost effective and useful tool for environmental monitoring.
Identifiers
External DOI: https://doi.org/10.1016/j.measen.2022.100368
This record's URL: https://www.repository.cam.ac.uk/handle/1810/335481
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