A Platform Approach to Protein Encapsulates with Controllable Surface Chemistry.
Publication Date
2022-03-28Journal Title
Molecules
ISSN
1420-3049
Publisher
MDPI AG
Volume
27
Issue
7
Language
en
Type
Article
This Version
VoR
Metadata
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Warner, N., Osojnik Črnivec, I. G., Rana, V. K., Cruz, M., & Scherman, O. (2022). A Platform Approach to Protein Encapsulates with Controllable Surface Chemistry.. Molecules, 27 (7) https://doi.org/10.3390/molecules27072197
Abstract
The encapsulation of proteins into core-shell structures is a widely utilised strategy for controlling protein stability, delivery and release. Despite the recognised utility of these microstructures, however, core-shell fabrication routes are often too costly or poorly scalable to allow for industrial translation. Furthermore, many scalable routes rely upon emulsion-techniques implicating denaturing or environmentally harmful organic solvents. Herein, we investigate core-shell protein encapsulation through single-feed, aqueous spray drying: a cheap, industrially ubiquitous particle-formation technology in the absence of organic solvents. We show that an excipient's preference for the surface of the spray dried particle is well-predicted by its hydrodynamic diameter (Dh) under relevant feed buffer conditions (pH and ionic strength) and that the predictive power of Dh is improved when measured at the spray dryer outlet temperature compared to room temperature (R2 = 0.64 vs. 0.59). Lastly, we leverage these findings to propose an adaptable design framework for fabricating core-shell protein encapsulates by single-feed aqueous spray drying.
Keywords
spray dry, protein, encapsulation, formulation, excipient, core-shell, EISA
Sponsorship
AB Agri (RG85712)
Slovenian Research Agency (ERC Fellowship 2019/3, J4-2545)
Identifiers
External DOI: https://doi.org/10.3390/molecules27072197
This record's URL: https://www.repository.cam.ac.uk/handle/1810/335541
Rights
Licence:
https://creativecommons.org/licenses/by/4.0/
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