CASP microdomain formation requires cross cell wall stabilization of domains and non-cell autonomous action of LOTR1.
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Authors
Marhavý, Peter
De Bellis, Damien
Li, Baohai
Kamiya, Takehiro
Kalmbach, Lothar
Publication Date
2022-01-14Journal Title
Elife
ISSN
2050-084X
Publisher
eLife Sciences Publications, Ltd
Volume
11
Number
ARTN e69602
Pages
e69602
Type
Article
This Version
VoR
Physical Medium
Electronic
Metadata
Show full item recordCitation
Kolbeck, A., Marhavý, P., De Bellis, D., Li, B., Kamiya, T., Fujiwara, T., Kalmbach, L., & et al. (2022). CASP microdomain formation requires cross cell wall stabilization of domains and non-cell autonomous action of LOTR1.. Elife, 11 (ARTN e69602), e69602. https://doi.org/10.7554/eLife.69602
Abstract
Efficient uptake of nutrients in both animal and plant cells requires tissue-spanning diffusion barriers separating inner tissues from the outer lumen/soil. However, we poorly understand how such contiguous three-dimensional superstructures are formed in plants. Here, we show that correct establishment of the plant Casparian Strip (CS) network relies on local neighbor communication. We show that positioning of Casparian Strip membrane domains (CSDs) is tightly coordinated between neighbors in wild-type and that restriction of domain formation involves the putative extracellular protease LOTR1. Impaired domain restriction in lotr1 leads to fully functional CSDs at ectopic positions, forming 'half strips'. LOTR1 action in the endodermis requires its expression in the stele. LOTR1 endodermal expression cannot complement, while cortex expression causes a dominant-negative phenotype. Our findings establish LOTR1 as a crucial player in CSD positioning acting in a directional, non-cell-autonomous manner to restrict and coordinate CS positioning.
Keywords
A. thaliana, arabidopsis, casparian strip, endodermis, microdomains, neprosin, network, plant biology, Arabidopsis, Arabidopsis Proteins, Cell Wall, Lignin, Promoter Regions, Genetic
Identifiers
External DOI: https://doi.org/10.7554/eLife.69602
This record's URL: https://www.repository.cam.ac.uk/handle/1810/335644
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