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dc.contributor.authorKolbeck, Andreas
dc.contributor.authorMarhavý, Peter
dc.contributor.authorDe Bellis, Damien
dc.contributor.authorLi, Baohai
dc.contributor.authorKamiya, Takehiro
dc.contributor.authorFujiwara, Toru
dc.contributor.authorKalmbach, Lothar
dc.contributor.authorGeldner, Niko
dc.date.accessioned2022-03-31T23:30:56Z
dc.date.available2022-03-31T23:30:56Z
dc.date.issued2022-01-14
dc.identifier.issn2050-084X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/335644
dc.description.abstractEfficient uptake of nutrients in both animal and plant cells requires tissue-spanning diffusion barriers separating inner tissues from the outer lumen/soil. However, we poorly understand how such contiguous three-dimensional superstructures are formed in plants. Here, we show that correct establishment of the plant Casparian Strip (CS) network relies on local neighbor communication. We show that positioning of Casparian Strip membrane domains (CSDs) is tightly coordinated between neighbors in wild-type and that restriction of domain formation involves the putative extracellular protease LOTR1. Impaired domain restriction in lotr1 leads to fully functional CSDs at ectopic positions, forming 'half strips'. LOTR1 action in the endodermis requires its expression in the stele. LOTR1 endodermal expression cannot complement, while cortex expression causes a dominant-negative phenotype. Our findings establish LOTR1 as a crucial player in CSD positioning acting in a directional, non-cell-autonomous manner to restrict and coordinate CS positioning.
dc.format.mediumElectronic
dc.publishereLife Sciences Publications, Ltd
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectA. thaliana
dc.subjectarabidopsis
dc.subjectcasparian strip
dc.subjectendodermis
dc.subjectmicrodomains
dc.subjectneprosin
dc.subjectnetwork
dc.subjectplant biology
dc.subjectArabidopsis
dc.subjectArabidopsis Proteins
dc.subjectCell Wall
dc.subjectLignin
dc.subjectPromoter Regions, Genetic
dc.titleCASP microdomain formation requires cross cell wall stabilization of domains and non-cell autonomous action of LOTR1.
dc.typeArticle
dc.publisher.departmentSainsbury Laboratory
dc.date.updated2022-03-31T08:55:05Z
prism.numberARTN e69602
prism.publicationDate2022
prism.publicationNameElife
prism.startingPagee69602
prism.volume11
dc.identifier.doi10.17863/CAM.83075
dcterms.dateAccepted2022-01-13
rioxxterms.versionofrecord10.7554/eLife.69602
rioxxterms.versionVoR
dc.contributor.orcidKolbeck, Andreas [0000-0001-9407-1958]
dc.contributor.orcidFujiwara, Toru [0000-0002-5363-6040]
dc.contributor.orcidGeldner, Niko [0000-0002-2300-9644]
dc.identifier.eissn2050-084X
rioxxterms.typeJournal Article/Review
cam.issuedOnline2022-01-14
cam.depositDate2022-03-31
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International