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dc.contributor.authorPrice, Stacey
dc.contributor.authorBhosle, Shriram
dc.contributor.authorGonçalves, Emanuel
dc.contributor.authorLi, Xiaodun
dc.contributor.authorMcClurg, Dylan P
dc.contributor.authorBarthorpe, Syd
dc.contributor.authorBeck, Alex
dc.contributor.authorHall, Caitlin
dc.contributor.authorLightfoot, Howard
dc.contributor.authorFarrow, Luke
dc.contributor.authorAnsari, Rizwan
dc.contributor.authorJackson, David A
dc.contributor.authorAllen, Laura
dc.contributor.authorRoberts, Kirsty
dc.contributor.authorBeaver, Charlotte
dc.contributor.authorFrancies, Hayley E
dc.contributor.authorGarnett, Mathew J
dc.date.accessioned2022-04-02T16:00:42Z
dc.date.available2022-04-02T16:00:42Z
dc.date.issued2022-04-02
dc.date.submitted2021-08-19
dc.identifier.issn2045-2322
dc.identifier.others41598-022-09508-y
dc.identifier.other9508
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/335708
dc.description.abstractOrganoid cell culture methodologies are enabling the generation of cell models from healthy and diseased tissue. Patient-derived cancer organoids that recapitulate the genetic and histopathological diversity of patient tumours are being systematically generated, providing an opportunity to investigate new cancer biology and therapeutic approaches. The use of organoid cultures for many applications, including genetic and chemical perturbation screens, is limited due to the technical demands and cost associated with their handling and propagation. Here we report and benchmark a suspension culture technique for cancer organoids which allows for the expansion of models to tens of millions of cells with increased efficiency in comparison to standard organoid culturing protocols. Using whole-genome DNA and RNA sequencing analyses, as well as medium-throughput drug sensitivity testing and genome-wide CRISPR-Cas9 screening, we demonstrate that cancer organoids grown as a suspension culture are genetically and phenotypically similar to their counterparts grown in standard conditions. This culture technique simplifies organoid cell culture and extends the range of organoid applications, including for routine use in large-scale perturbation screens.
dc.languageen
dc.publisherSpringer Science and Business Media LLC
dc.subjectArticle
dc.subject/631/80
dc.subject/631/154
dc.subject/631/208
dc.subject/631/67
dc.subject/631/67/69
dc.subject/631/67/70
dc.subjectarticle
dc.titleA suspension technique for efficient large-scale cancer organoid culturing and perturbation screens.
dc.typeArticle
dc.date.updated2022-04-02T16:00:41Z
prism.issueIdentifier1
prism.publicationNameSci Rep
prism.volume12
dc.identifier.doi10.17863/CAM.83143
dcterms.dateAccepted2022-03-15
rioxxterms.versionofrecord10.1038/s41598-022-09508-y
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.identifier.eissn2045-2322
pubs.funder-project-idWellcome Trust (206194, 206194, 206194, 206194, 206194, 206194, 206194, 206194, 206194, 206194, 206194, 206194, 206194, 206194, 206194)
pubs.funder-project-idMedical Research Council (RG84369, RG84369)
cam.issuedOnline2022-04-02


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