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Multi-omic rejuvenation of naturally aged tissues by a single cycle of transient reprogramming.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Chondronasiou, Dafni 
Berenguer-Llergo, Antonio  ORCID logo  https://orcid.org/0000-0002-3742-8161

Abstract

The expression of the pluripotency factors OCT4, SOX2, KLF4, and MYC (OSKM) can convert somatic differentiated cells into pluripotent stem cells in a process known as reprogramming. Notably, partial and reversible reprogramming does not change cell identity but can reverse markers of aging in cells, improve the capacity of aged mice to repair tissue injuries, and extend longevity in progeroid mice. However, little is known about the mechanisms involved. Here, we have studied changes in the DNA methylome, transcriptome, and metabolome in naturally aged mice subject to a single period of transient OSKM expression. We found that this is sufficient to reverse DNA methylation changes that occur upon aging in the pancreas, liver, spleen, and blood. Similarly, we observed reversion of transcriptional changes, especially regarding biological processes known to change during aging. Finally, some serum metabolites and biomarkers altered with aging were also restored to young levels upon transient reprogramming. These observations indicate that a single period of OSKM expression can drive epigenetic, transcriptomic, and metabolomic changes toward a younger configuration in multiple tissues and in the serum.

Description

Keywords

OSKM, Yamanaka, aging, epigenetic clocks, pluripotency, reprogramming, transcriptomic clocks, Animals, Cell Differentiation, Cellular Reprogramming, DNA Methylation, Epigenome, Induced Pluripotent Stem Cells, Mice, Rejuvenation

Journal Title

Aging Cell

Conference Name

Journal ISSN

1474-9718
1474-9726

Volume Title

21

Publisher

Wiley
Sponsorship
Fundación Científica Asociación Española Contra el Cáncer (PROYE18061FERN)
European Research Council (ERC‐2014‐AdG/669622, ERC-2014-AdG/669622)
Ministerio de Ciencia e Innovación (SAF2013-48256-R, SAF2013‐48256‐R)
Biotechnology and Biological Sciences Research Council (BBS/E/B/000C0425)