Show simple item record

dc.contributor.authorChondronasiou, Dafni
dc.contributor.authorGill, Diljeet
dc.contributor.authorMosteiro, Lluc
dc.contributor.authorUrdinguio, Rocio G
dc.contributor.authorBerenguer-Llergo, Antonio
dc.contributor.authorAguilera, Mònica
dc.contributor.authorDurand, Sylvere
dc.contributor.authorAprahamian, Fanny
dc.contributor.authorNirmalathasan, Nitharsshini
dc.contributor.authorAbad, Maria
dc.contributor.authorMartin-Herranz, Daniel E
dc.contributor.authorStephan-Otto Attolini, Camille
dc.contributor.authorPrats, Neus
dc.contributor.authorKroemer, Guido
dc.contributor.authorFraga, Mario F
dc.contributor.authorReik, Wolf
dc.contributor.authorSerrano, Manuel
dc.date.accessioned2022-04-03T01:02:45Z
dc.date.available2022-04-03T01:02:45Z
dc.date.issued2022-03
dc.identifier.issn1474-9718
dc.identifier.other35235716
dc.identifier.otherPMC8920440
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/335717
dc.description.abstractThe expression of the pluripotency factors OCT4, SOX2, KLF4, and MYC (OSKM) can convert somatic differentiated cells into pluripotent stem cells in a process known as reprogramming. Notably, partial and reversible reprogramming does not change cell identity but can reverse markers of aging in cells, improve the capacity of aged mice to repair tissue injuries, and extend longevity in progeroid mice. However, little is known about the mechanisms involved. Here, we have studied changes in the DNA methylome, transcriptome, and metabolome in naturally aged mice subject to a single period of transient OSKM expression. We found that this is sufficient to reverse DNA methylation changes that occur upon aging in the pancreas, liver, spleen, and blood. Similarly, we observed reversion of transcriptional changes, especially regarding biological processes known to change during aging. Finally, some serum metabolites and biomarkers altered with aging were also restored to young levels upon transient reprogramming. These observations indicate that a single period of OSKM expression can drive epigenetic, transcriptomic, and metabolomic changes toward a younger configuration in multiple tissues and in the serum.
dc.languageeng
dc.publisherWiley
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcenlmid: 101130839
dc.sourceessn: 1474-9726
dc.subjectAging
dc.subjectPluripotency
dc.subjectReprogramming
dc.subjectOskm
dc.subjectYamanaka
dc.subjectEpigenetic Clocks
dc.subjectTranscriptomic Clocks
dc.titleMulti-omic rejuvenation of naturally aged tissues by a single cycle of transient reprogramming.
dc.typeArticle
dc.date.updated2022-04-03T01:02:45Z
prism.issueIdentifier3
prism.publicationNameAging Cell
prism.volume21
dc.identifier.doi10.17863/CAM.83152
dcterms.dateAccepted2022-02-09
rioxxterms.versionofrecord10.1111/acel.13578
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidGill, Diljeet [0000-0002-5725-2466]
dc.contributor.orcidMosteiro, Lluc [0000-0003-4041-9706]
dc.contributor.orcidUrdinguio, Rocio G [0000-0003-2736-5758]
dc.contributor.orcidBerenguer-Llergo, Antonio [0000-0002-3742-8161]
dc.contributor.orcidAguilera, Mònica [0000-0003-0763-7947]
dc.contributor.orcidAbad, Maria [0000-0002-1462-2498]
dc.contributor.orcidMartin-Herranz, Daniel E [0000-0002-2285-3317]
dc.contributor.orcidStephan-Otto Attolini, Camille [0000-0001-8045-320X]
dc.contributor.orcidPrats, Neus [0000-0001-5653-7390]
dc.contributor.orcidFraga, Mario F [0000-0001-8450-2603]
dc.contributor.orcidReik, Wolf [0000-0003-0216-9881]
dc.contributor.orcidSerrano, Manuel [0000-0001-7177-9312]
dc.identifier.eissn1474-9726
pubs.funder-project-idFundación Científica Asociación Española Contra el Cáncer (PROYE18061FERN)
pubs.funder-project-idEuropean Research Council (ERC‐2014‐AdG/669622, ERC-2014-AdG/669622)
pubs.funder-project-idMinisterio de Ciencia e Innovación (SAF2013-48256-R, SAF2013‐48256‐R)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BBS/E/B/000C0425)
cam.issuedOnline2022-03-02


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International