Microfluidic platforms for single neuron analysis.
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Authors
Gupta, Pallavi
Shinde, Ashwini
Illath, Kavitha
Kar, Srabani
Nagai, Moeto
Tseng, Fan-Gang
Publication Date
2022-01Journal Title
Mater Today Bio
ISSN
2590-0064
Publisher
Elsevier BV
Volume
13
Language
eng
Type
Article
This Version
VoR
Metadata
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Gupta, P., Shinde, A., Illath, K., Kar, S., Nagai, M., Tseng, F., & Santra, T. S. (2022). Microfluidic platforms for single neuron analysis.. Mater Today Bio, 13 https://doi.org/10.1016/j.mtbio.2022.100222
Abstract
Single-neuron actions are the basis of brain function, as clinical sequelae, neuronal dysfunction or failure for most of the central nervous system (CNS) diseases and injuries can be identified via tracing single-neurons. The bulk analysis methods tend to miscue critical information by assessing the population-averaged outcomes. However, its primary requisite in neuroscience to analyze single-neurons and to understand dynamic interplay of neurons and their environment. Microfluidic systems enable precise control over nano-to femto-liter volumes via adjusting device geometry, surface characteristics, and flow-dynamics, thus facilitating a well-defined micro-environment with spatio-temporal control for single-neuron analysis. The microfluidic platform not only offers a comprehensive landscape to study brain cell diversity at the level of transcriptome, genome, and/or epigenome of individual cells but also has a substantial role in deciphering complex dynamics of brain development and brain-related disorders. In this review, we highlight recent advances of microfluidic devices for single-neuron analysis, i.e., single-neuron trapping, single-neuron dynamics, single-neuron proteomics, single-neuron transcriptomics, drug delivery at the single-neuron level, single axon guidance, and single-neuron differentiation. Moreover, we also emphasize limitations and future challenges of single-neuron analysis by focusing on key performances of throughput and multiparametric activity analysis on microfluidic platforms.
Keywords
Microelectrode Array, Single Cell Analysis, Omics, Microfluidic Devices, Single Neuron Analysis, Single Axon Guidance, Single Neuron Dynamics
Identifiers
35243297, PMC8866890
External DOI: https://doi.org/10.1016/j.mtbio.2022.100222
This record's URL: https://www.repository.cam.ac.uk/handle/1810/335725
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International
Licence URL: https://creativecommons.org/licenses/by-nc-nd/4.0/
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