Clamping of DNA shuts the condensin neck gate.
Proc Natl Acad Sci U S A
Proceedings of the National Academy of Sciences
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Lee, B., Rhodes, J., & Löwe, J. (2022). Clamping of DNA shuts the condensin neck gate.. Proc Natl Acad Sci U S A, 119 (14) https://doi.org/10.1073/pnas.2120006119
SignificanceDNA needs to be compacted to fit into nuclei and during cell division, when dense chromatids are formed for their mechanical segregation, a process that depends on the protein complex condensin. It forms and enlarges loops in DNA through loop extrusion. Our work resolves the atomic structure of a DNA-bound state of condensin in which ATP has not been hydrolyzed. The DNA is clamped within a compartment that has been reported previously in other structural maintenance of chromosomes (SMC) complexes, including Rad50, cohesin, and MukBEF. With the caveat of important differences, it means that all SMC complexes cycle through at least some similar states and undergo similar conformational changes in their head modules, while hydrolyzing ATP and translocating DNA.
408, Biological Sciences, SMC complex, cryo-EM, loop extrusion, DNA motor
RCUK | Medical Research Council (MRC) (U105184326)
External DOI: https://doi.org/10.1073/pnas.2120006119
This record's URL: https://www.repository.cam.ac.uk/handle/1810/335798