NanoDam identifies Homeobrain (ARX) and Scarecrow (NKX2.1) as conserved temporal factors in the Drosophila central brain and visual system
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Authors
Tang, Jocelyn LY
Hakes, Anna E
Krautz, Robert
Suzuki, Takumi
Contreras, Esteban G
Fox, Paul M
Publication Date
2022-05-09Journal Title
Developmental Cell
ISSN
1534-5807
Publisher
Elsevier
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Brand, A., Tang, J. L., Hakes, A. E., Krautz, R., Suzuki, T., Contreras, E. G., & Fox, P. M. (2022). NanoDam identifies Homeobrain (ARX) and Scarecrow (NKX2.1) as conserved temporal factors in the Drosophila central brain and visual system. Developmental Cell https://doi.org/10.1016/j.devcel.2022.04.008
Abstract
Temporal patterning of neural progenitors is an evolutionarily conserved strategy for generating neuronal diversity. Type II neural stem cells in the Drosophila central brain produce transit-amplifying intermediate neural progenitors (INPs) that exhibit temporal patterning. However, the known temporal factors cannot account for the neuronal diversity in the adult brain. To search for missing factors, we developed NanoDam, enabling rapid genome-wide profiling of endogenously tagged proteins in vivo with a single genetic cross. Mapping the targets of known temporal transcription factors with NanoDam revealed that Homeobrain and Scarecrow (ARX and NKX2.1 orthologues) are also temporal factors. We show that Homeobrain and Scarecrow define middle-aged and late INP temporal windows and play a role in cellular longevity. Strikingly, Homeobrain and Scarecrow have conserved functions as temporal factors in the developing visual system. NanoDam enables rapid cell type specific genome-wide profiling with temporal resolution and is easily adapted for use in higher organisms.
Sponsorship
Wellcome Trust (092545/Z/10/Z)
Wellcome Trust (103792/Z/14/Z)
Royal Society (RP150061)
Wellcome Trust (089616/Z/09/Z)
Wellcome Trust (092096/Z/10/Z)
Cancer Research Uk (None)
Wellcome Trust (102454/Z/13/Z)
Wellcome Trust (203798/Z/16/Z)
Identifiers
External DOI: https://doi.org/10.1016/j.devcel.2022.04.008
This record's URL: https://www.repository.cam.ac.uk/handle/1810/335851
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