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dc.contributor.authorBrand, Andrea
dc.contributor.authorTang, Jocelyn LY
dc.contributor.authorHakes, Anna E
dc.contributor.authorKrautz, Robert
dc.contributor.authorSuzuki, Takumi
dc.contributor.authorContreras, Esteban G
dc.contributor.authorFox, Paul M
dc.date.accessioned2022-04-06T23:30:48Z
dc.date.available2022-04-06T23:30:48Z
dc.date.issued2022-05-09
dc.identifier.issn1534-5807
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/335851
dc.description.abstractTemporal patterning of neural progenitors is an evolutionarily conserved strategy for generating neuronal diversity. Type II neural stem cells in the Drosophila central brain produce transit-amplifying intermediate neural progenitors (INPs) that exhibit temporal patterning. However, the known temporal factors cannot account for the neuronal diversity in the adult brain. To search for missing factors, we developed NanoDam, enabling rapid genome-wide profiling of endogenously tagged proteins in vivo with a single genetic cross. Mapping the targets of known temporal transcription factors with NanoDam revealed that Homeobrain and Scarecrow (ARX and NKX2.1 orthologues) are also temporal factors. We show that Homeobrain and Scarecrow define middle-aged and late INP temporal windows and play a role in cellular longevity. Strikingly, Homeobrain and Scarecrow have conserved functions as temporal factors in the developing visual system. NanoDam enables rapid cell type specific genome-wide profiling with temporal resolution and is easily adapted for use in higher organisms.
dc.publisherElsevier
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleNanoDam identifies Homeobrain (ARX) and Scarecrow (NKX2.1) as conserved temporal factors in the Drosophila central brain and visual system
dc.typeArticle
dc.publisher.departmentDepartment of Physiology, Development And Neuroscience
dc.date.updated2022-04-06T13:18:48Z
prism.publicationNameDevelopmental Cell
dc.identifier.doi10.17863/CAM.83284
dcterms.dateAccepted2022-04-05
rioxxterms.versionofrecord10.1016/j.devcel.2022.04.008
rioxxterms.versionVoR
dc.contributor.orcidBrand, Andrea [0000-0002-2089-6954]
dc.identifier.eissn1878-1551
dc.publisher.urlhttps://www.sciencedirect.com/science/article/pii/S1534580722002489?via%3Dihub#!
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (092545/Z/10/Z)
pubs.funder-project-idWellcome Trust (103792/Z/14/Z)
pubs.funder-project-idRoyal Society (RP150061)
pubs.funder-project-idWellcome Trust (089616/Z/09/Z)
pubs.funder-project-idWellcome Trust (092096/Z/10/Z)
pubs.funder-project-idCancer Research Uk (None)
pubs.funder-project-idWellcome Trust (102454/Z/13/Z)
pubs.funder-project-idWellcome Trust (203798/Z/16/Z)
cam.issuedOnline2022-05-09
cam.depositDate2022-04-06
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International