SARS-CoV-2 Spike Protein Binding of Glycated Serum Albumin-Its Potential Role in the Pathogenesis of the COVID-19 Clinical Syndromes and Bias towards Individuals with Pre-Diabetes/Type 2 Diabetes and Metabolic Diseases.
Authors
Iles, Jason
Gardiner, Anna
Lacey, Jonathan
Harding, Stephen
Ule, Jernej
Roblett, Debra
Heeney, Jonathan
Baxendale, Helen
Publication Date
2022-04-08Journal Title
Int J Mol Sci
ISSN
1661-6596
Publisher
MDPI AG
Volume
23
Issue
8
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Iles, J., Zmuidinaite, R., Sadee, C., Gardiner, A., Lacey, J., Harding, S., Ule, J., et al. (2022). SARS-CoV-2 Spike Protein Binding of Glycated Serum Albumin-Its Potential Role in the Pathogenesis of the COVID-19 Clinical Syndromes and Bias towards Individuals with Pre-Diabetes/Type 2 Diabetes and Metabolic Diseases.. Int J Mol Sci, 23 (8) https://doi.org/10.3390/ijms23084126
Abstract
The immune response to SARS-CoV-2 infection requires antibody recognition of the spike protein. In a study designed to examine the molecular features of anti-spike and anti-nucleocapsid antibodies, patient plasma proteins binding to pre-fusion stabilised complete spike and nucleocapsid proteins were isolated and analysed by matrix-assisted laser desorption ionisation-time of flight (MALDI-ToF) mass spectrometry. Amongst the immunoglobulins, a high affinity for human serum albumin was evident in the anti-spike preparations. Careful mass comparison revealed the preferential capture of advanced glycation end product (AGE) forms of glycated human serum albumin by the pre-fusion spike protein. The ability of bacteria and viruses to surround themselves with serum proteins is a recognised immune evasion and pathogenic process. The preference of SARS-CoV-2 for AGE forms of glycated serum albumin may in part explain the severity and pathology of acute respiratory distress and the bias towards the elderly and those with (pre)diabetic and atherosclerotic/metabolic disease.
Keywords
COVID-19, convalescent plasma, spike protein, nucleocapsid, glycated albumin, semi-automated magnetic rack
Sponsorship
National Institute for Health Research (G107217)
Identifiers
External DOI: https://doi.org/10.3390/ijms23084126
This record's URL: https://www.repository.cam.ac.uk/handle/1810/335958
Rights
Licence:
https://creativecommons.org/licenses/by/4.0/
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