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dc.contributor.authorIbrahim, Iskander M
dc.contributor.authorRowden, Stephen JL
dc.contributor.authorCramer, William A
dc.contributor.authorHowe, Christopher J
dc.contributor.authorPuthiyaveetil, Sujith
dc.date.accessioned2022-04-11T12:00:10Z
dc.date.available2022-04-11T12:00:10Z
dc.date.issued2022-06
dc.date.submitted2022-02-18
dc.identifier.issn0014-5793
dc.identifier.otherfeb214340
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/335980
dc.descriptionFunder: Spicer Consulting Ltd
dc.description.abstractCyanobacteria employ two-component sensor-response regulator systems to monitor and respond to environmental challenges. The response regulators RpaA, RpaB, Rre1 and RppA are integral to circadian clock function and abiotic stress acclimation in cyanobacteria. RpaA, RpaB and Rre1 are known to interact with ferredoxin or thioredoxin, raising the possibility of their thiol regulation. Here, we report that Synechocystis sp. PCC 6803 Rre1, RpaA and RpaB exist as higher-order oligomers under oxidising conditions and that reduced thioredoxin A converts them to monomers. We further show that these response regulators contain redox-responsive cysteine residues with an Em7 around -300 mV. These findings suggest a direct thiol modulation of the activity of these response regulators, independent of their cognate sensor kinases.
dc.languageen
dc.publisherWiley
dc.subjectResearch Letter
dc.subjectResearch Letters
dc.subjectHik2
dc.subjectRpaA
dc.subjectRpaB
dc.subjectRre1
dc.subjectthiol regulation
dc.subjectTrxA
dc.titleThiol redox switches regulate the oligomeric state of cyanobacterial Rre1, RpaA and RpaB response regulators.
dc.typeArticle
dc.date.updated2022-04-11T12:00:10Z
prism.publicationNameFEBS Lett
dc.identifier.doi10.17863/CAM.83412
dcterms.dateAccepted2022-03-16
rioxxterms.versionofrecord10.1002/1873-3468.14340
rioxxterms.versionAO
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidIbrahim, Iskander M [0000-0002-2744-6307]
dc.identifier.eissn1873-3468
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/F047940/1)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/L014130/1)
pubs.funder-project-idBBSRC (BB/T010525/1)
cam.issuedOnline2022-04-11


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