DGAT1 activity synchronises with mitophagy to protect cells from metabolic rewiring by iron depletion.
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Long, M., Sanchez-Martinez, A., Longo, M., Suomi, F., Stenlund, H., Johansson, A. I., Ehsan, H., et al. (2022). DGAT1 activity synchronises with mitophagy to protect cells from metabolic rewiring by iron depletion.. EMBO J https://doi.org/10.15252/embj.2021109390
Funder: Novo Nordisk Foundation/Novo Nordisk Fonden; Id: http://dx.doi.org/10.13039/501100011747
Funder: Helsingin Yliopisto (HY); Id: http://dx.doi.org/10.13039/100007797
Funder: Sydäntutkimussäätiö (Finnish Foundation for Cardiovascular Research); Id: http://dx.doi.org/10.13039/501100005633
Funder: Jane and Aatos Erkko Foundation; Id: http://dx.doi.org/10.13039/501100004012
Mitophagy removes defective mitochondria via lysosomal elimination. Increased mitophagy coincides with metabolic reprogramming, yet it remains unknown whether mitophagy is a cause or consequence of such state changes. The signalling pathways that integrate with mitophagy to sustain cell and tissue integrity also remain poorly defined. We performed temporal metabolomics on mammalian cells treated with deferiprone, a therapeutic iron chelator that stimulates PINK1/PARKIN-independent mitophagy. Iron depletion profoundly rewired the metabolome, hallmarked by remodelling of lipid metabolism within minutes of treatment. DGAT1-dependent lipid droplet biosynthesis occurred several hours before mitochondrial clearance, with lipid droplets bordering mitochondria upon iron chelation. We demonstrate that DGAT1 inhibition restricts mitophagy in vitro, with impaired lysosomal homeostasis and cell viability. Importantly, genetic depletion of DGAT1 in vivo significantly impaired neuronal mitophagy and locomotor function in Drosophila. Our data define iron depletion as a potent signal that rapidly reshapes metabolism and establishes an unexpected synergy between lipid homeostasis and mitophagy that safeguards cell and tissue integrity.
EMBO07, EMBO21, Article, Articles, DGAT1, iron, lipid droplet, metabolism, mitophagy
Medical Research Council (MC_UU_00015/6)
External DOI: https://doi.org/10.15252/embj.2021109390
This record's URL: https://www.repository.cam.ac.uk/handle/1810/336022