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dc.contributor.authorDamm, Elena
dc.contributor.authorUllrich, Kristian K
dc.contributor.authorAmos, William B
dc.contributor.authorOdenthal-Hesse, Linda
dc.date.accessioned2022-04-19T13:17:01Z
dc.date.available2022-04-19T13:17:01Z
dc.date.issued2022-03-16
dc.identifier.issn1471-2164
dc.identifier.other35296233
dc.identifier.otherPMC8925151
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/336156
dc.descriptionFunder: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.
dc.description.abstractBACKGROUND: PRDM9 is a key regulator of meiotic recombination in most metazoans, responsible for reshuffling parental genomes. During meiosis, the PRDM9 protein recognizes and binds specific target motifs via its array of C2H2 zinc-fingers encoded by a rapidly evolving minisatellite. The gene coding for PRDM9 is the only speciation gene identified in vertebrates to date and shows high variation, particularly in the DNA-recognizing positions of the zinc-finger array, within and between species. Across all vertebrate genomes studied for PRDM9 evolution, only one genome lacks variability between repeat types - that of the North Pacific minke whale. This study aims to understand the evolution and diversity of Prdm9 in minke whales, which display the most unusual genome reference allele of Prdm9 so far discovered in mammals. RESULTS: Minke whales possess all the features characteristic of PRDM9-directed recombination, including complete KRAB, SSXRD and SET domains and a rapidly evolving array of C2H2-type-Zincfingers (ZnF) with evidence of rapid evolution, particularly at DNA-recognizing positions that evolve under positive diversifying selection. Seventeen novel PRDM9 variants were identified within the Antarctic minke whale species, plus a single distinct PRDM9 variant in Common minke whales - shared across North Atlantic and North Pacific minke whale subspecies boundaries. CONCLUSION: The PRDM9 ZnF array evolves rapidly, in minke whales, with at least one DNA-recognizing position under positive selection. Extensive PRDM9 diversity is observed, particularly in the Antarctic in minke whales. Common minke whales shared a specific Prdm9 allele across subspecies boundaries, suggesting incomplete speciation by the mechanisms associated with PRDM9 hybrid sterility.
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcenlmid: 100965258
dc.sourceessn: 1471-2164
dc.subjectmtDNA
dc.subjectMicrosatellite loci
dc.subjectPrdm9
dc.subjectBalaenoptera Bonaerensis
dc.subjectMinke Whales
dc.subjectPostzygotic Reproductive Isolation
dc.subjectBalaenoptera Acutorostrata
dc.subjectMeiotic Recombination Regulation
dc.subjectAnimals
dc.subjectHistone-Lysine N-Methyltransferase
dc.subjectMeiosis
dc.subjectZinc Fingers
dc.subjectAlleles
dc.subjectMinke Whale
dc.titleEvolution of the recombination regulator PRDM9 in minke whales.
dc.typeArticle
dc.date.updated2022-04-19T13:17:01Z
prism.issueIdentifier1
prism.publicationNameBMC Genomics
prism.volume23
dc.identifier.doi10.17863/CAM.83581
dcterms.dateAccepted2022-01-11
rioxxterms.versionofrecord10.1186/s12864-022-08305-1
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidDamm, Elena [0000-0002-6261-6076]
dc.contributor.orcidUllrich, Kristian K [0000-0003-4308-9626]
dc.contributor.orcidOdenthal-Hesse, Linda [0000-0002-5519-2375]
dc.identifier.eissn1471-2164
cam.issuedOnline2022-03-16


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International