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dc.contributor.authorPerdikari, Aliki
dc.contributor.authorCacciottolo, Tessa
dc.contributor.authorHenning, Elana
dc.contributor.authorMendes de Oliveira, Edson
dc.contributor.authorKeogh, Julia M
dc.contributor.authorFarooqi, I Sadaf
dc.date.accessioned2022-04-19T13:17:07Z
dc.date.available2022-04-19T13:17:07Z
dc.date.issued2022-03
dc.identifier.issn2046-2441
dc.identifier.other35291877
dc.identifier.otherPMC8924755
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/336158
dc.descriptionFunder: Botnar Fondation
dc.descriptionFunder: NIHR Senior Investigator Award
dc.descriptionFunder: Bernard Wolfe Health Neuroscience Endowment
dc.descriptionFunder: NIHR Cambridge Biomedical Research Center
dc.description.abstractObesity, defined as an excess of adipose tissue that adversely affects health, is a major cause of morbidity and mortality. However, to date, understanding the structure and function of human adipose tissue has been limited by the inability to visualize cellular components due to the innate structure of adipocytes, which are characterized by large lipid droplets. Combining the iDISCO and the CUBIC protocols for whole tissue staining and optical clearing, we developed a protocol to enable immunostaining and clearing of human subcutaneous white adipose tissue (WAT) obtained from individuals with severe obesity. We were able to perform immunolabelling of sympathetic nerve terminals in whole WAT and subsequent optical clearing by eliminating lipids to render the opaque tissue completely transparent. We then used light sheet confocal microscopy to visualize sympathetic innervation of human WAT from obese individuals in a three-dimensional manner. We demonstrate the visualization of sympathetic nerve terminals in human WAT. This protocol can be modified to visualize other structures such as blood vessels involved in the development, maintenance and function of human adipose tissue in health and disease.
dc.languageeng
dc.publisherThe Royal Society
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcenlmid: 101580419
dc.sourceessn: 2046-2441
dc.subjectObesity
dc.subjectHuman Adipose Tissue
dc.subjectSympathetic Innervation
dc.subjectThree-dimensional Microscopy
dc.subjectWhole Tissue Immunolabelling
dc.titleVisualization of sympathetic neural innervation in human white adipose tissue.
dc.typeArticle
dc.date.updated2022-04-19T13:17:07Z
prism.issueIdentifier3
prism.publicationNameOpen Biol
prism.volume12
dc.identifier.doi10.17863/CAM.83583
dcterms.dateAccepted2022-02-08
rioxxterms.versionofrecord10.1098/rsob.210345
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidPerdikari, Aliki [0000-0001-6841-4187]
dc.contributor.orcidMendes de Oliveira, Edson [0000-0002-7330-7826]
dc.contributor.orcidFarooqi, I Sadaf [0000-0001-7609-3504]
dc.identifier.eissn2046-2441
pubs.funder-project-idWellcome Trust (207462/Z/17/Z)
pubs.funder-project-idWellcome Trust (208363/Z/17/Z)
cam.issuedOnline2022-03-16


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International