An Overview of Cryo-Scanning Electron Microscopy Techniques for Plant Imaging.
Authors
Publication Date
2022-04-20Journal Title
Plants (Basel)
ISSN
2223-7747
Publisher
MDPI AG
Volume
11
Issue
9
Language
en
Type
Article
This Version
VoR
Metadata
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Wightman, R. (2022). An Overview of Cryo-Scanning Electron Microscopy Techniques for Plant Imaging.. Plants (Basel), 11 (9) https://doi.org/10.3390/plants11091113
Abstract
Many research questions require the study of plant morphology, in particular cells and tissues, as close to their native context as possible and without physical deformations from some preparatory chemical reagents or sample drying. Cryo-scanning electron microscopy (cryoSEM) involves rapid freezing and maintenance of the sample at an ultra-low temperature for detailed surface imaging by a scanning electron beam. The data are useful for exploring tissue/cell morphogenesis, plus an additional cryofracture/cryoplaning/milling step gives information on air and water spaces as well as subcellular ultrastructure. This review gives an overview from sample preparation through to imaging and a detailed account of how this has been applied across diverse areas of plant research. Future directions and improvements to the technique are discussed.
Keywords
cryo, scanning electron microscopy, sample preparation, hydration, plant–microbe interactions, cell wall, plant patterning, membranes, organelles
Sponsorship
Gatsby Charitable Foundation
Identifiers
External DOI: https://doi.org/10.3390/plants11091113
This record's URL: https://www.repository.cam.ac.uk/handle/1810/336358
Rights
Licence:
https://creativecommons.org/licenses/by/4.0/
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