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dc.contributor.authorWightman, Raymond
dc.date.accessioned2022-04-22T02:30:44Z
dc.date.available2022-04-22T02:30:44Z
dc.date.issued2022-04-20
dc.identifier.issn2223-7747
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/336358
dc.description.abstractMany research questions require the study of plant morphology, in particular cells and tissues, as close to their native context as possible and without physical deformations from some preparatory chemical reagents or sample drying. Cryo-scanning electron microscopy (cryoSEM) involves rapid freezing and maintenance of the sample at an ultra-low temperature for detailed surface imaging by a scanning electron beam. The data are useful for exploring tissue/cell morphogenesis, plus an additional cryofracture/cryoplaning/milling step gives information on air and water spaces as well as subcellular ultrastructure. This review gives an overview from sample preparation through to imaging and a detailed account of how this has been applied across diverse areas of plant research. Future directions and improvements to the technique are discussed.
dc.description.sponsorshipGatsby Charitable Foundation
dc.languageen
dc.publisherMDPI AG
dc.subjectcryo
dc.subjectscanning electron microscopy
dc.subjectsample preparation
dc.subjecthydration
dc.subjectplant–microbe interactions
dc.subjectcell wall
dc.subjectplant patterning
dc.subjectmembranes
dc.subjectorganelles
dc.titleAn Overview of Cryo-Scanning Electron Microscopy Techniques for Plant Imaging.
dc.typeArticle
dc.date.updated2022-04-22T02:30:43Z
prism.issueIdentifier9
prism.publicationNamePlants (Basel)
prism.volume11
dc.identifier.doi10.17863/CAM.83778
dcterms.dateAccepted2022-04-14
rioxxterms.versionofrecord10.3390/plants11091113
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidWightman, Raymond [0000-0003-1295-4875]
dc.identifier.eissn2223-7747
cam.issuedOnline2022-04-20


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