Show simple item record

dc.contributor.authorStewart-McGuinness, Callum
dc.contributor.authorPlatt, Christopher I
dc.contributor.authorOzols, Matiss
dc.contributor.authorGoh, Brian
dc.contributor.authorGriffiths, Tamara W
dc.contributor.authorSherratt, Michael J
dc.date.accessioned2022-04-25T01:03:02Z
dc.date.available2022-04-25T01:03:02Z
dc.date.issued2022-03-20
dc.identifier.issn2218-273X
dc.identifier.other35327667
dc.identifier.otherPMC8946613
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/336408
dc.description.abstractProteases and protease inhibitors (P/PIs) are involved in many biological processes in human skin, yet often only specific families or related groups of P/PIs are investigated. Proteomics approaches, such as mass spectrometry, can define proteome signatures (including P/PIs) in tissues; however, they struggle to detect low-abundance proteins. To overcome these issues, we aimed to produce a comprehensive proteome of all P/PIs present in normal and diseased human skin, in vivo, by carrying out a modified systematic review using a list of P/PIs from MEROPS and combining this with key search terms in Web of Science. Resulting articles were manually reviewed against inclusion/exclusion criteria and a dataset constructed. This study identified 111 proteases and 77 protease inhibitors in human skin, comprising the serine, metallo-, cysteine and aspartic acid catalytic families of proteases. P/PIs showing no evidence of catalytic activity or protease inhibition, were designated non-peptidase homologs (NPH), and no reported protease inhibitory activity (NRPIA), respectively. MMP9 and TIMP1 were the most frequently published P/PIs and were reported in normal skin and most skin disease groups. Normal skin and diseased skin showed significant overlap with respect to P/PI profile; however, MMP23 was identified in several skin disease groups, but was absent in normal skin. The catalytic profile of P/PIs in wounds, scars and solar elastosis was distinct from normal skin, suggesting that a different group of P/PIs is responsible for disease progression. In conclusion, this study uses a novel approach to provide a comprehensive inventory of P/PIs in normal and diseased human skin reported in our database. The database may be used to determine either which P/PIs are present in specific diseases or which diseases individual P/PIs may influence.
dc.languageeng
dc.publisherMDPI AG
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcenlmid: 101596414
dc.sourceessn: 2218-273X
dc.subjectHuman
dc.subjectProtease
dc.subjectProteome
dc.subjectProtease inhibitor
dc.subjectSKin
dc.titleDefining the Protease and Protease Inhibitor (P/PI) Proteomes of Healthy and Diseased Human Skin by Modified Systematic Review.
dc.typeArticle
dc.date.updated2022-04-25T01:03:02Z
prism.issueIdentifier3
prism.publicationNameBiomolecules
prism.volume12
dc.identifier.doi10.17863/CAM.83825
dcterms.dateAccepted2022-03-18
rioxxterms.versionofrecord10.3390/biom12030475
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidStewart-McGuinness, Callum [0000-0002-1572-4402]
dc.contributor.orcidPlatt, Christopher I [0000-0002-0525-3221]
dc.contributor.orcidOzols, Matiss [0000-0001-5663-1053]
dc.contributor.orcidGoh, Brian [0000-0003-2253-1331]
dc.contributor.orcidSherratt, Michael J [0000-0003-4759-6617]
dc.identifier.eissn2218-273X
cam.issuedOnline2022-03-20


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International