Defining the Protease and Protease Inhibitor (P/PI) Proteomes of Healthy and Diseased Human Skin by Modified Systematic Review.
dc.contributor.author | Stewart-McGuinness, Callum | |
dc.contributor.author | Platt, Christopher I | |
dc.contributor.author | Ozols, Matiss | |
dc.contributor.author | Goh, Brian | |
dc.contributor.author | Griffiths, Tamara W | |
dc.contributor.author | Sherratt, Michael J | |
dc.date.accessioned | 2022-04-25T01:03:02Z | |
dc.date.available | 2022-04-25T01:03:02Z | |
dc.date.issued | 2022-03-20 | |
dc.identifier.issn | 2218-273X | |
dc.identifier.other | 35327667 | |
dc.identifier.other | PMC8946613 | |
dc.identifier.uri | https://www.repository.cam.ac.uk/handle/1810/336408 | |
dc.description.abstract | Proteases and protease inhibitors (P/PIs) are involved in many biological processes in human skin, yet often only specific families or related groups of P/PIs are investigated. Proteomics approaches, such as mass spectrometry, can define proteome signatures (including P/PIs) in tissues; however, they struggle to detect low-abundance proteins. To overcome these issues, we aimed to produce a comprehensive proteome of all P/PIs present in normal and diseased human skin, in vivo, by carrying out a modified systematic review using a list of P/PIs from MEROPS and combining this with key search terms in Web of Science. Resulting articles were manually reviewed against inclusion/exclusion criteria and a dataset constructed. This study identified 111 proteases and 77 protease inhibitors in human skin, comprising the serine, metallo-, cysteine and aspartic acid catalytic families of proteases. P/PIs showing no evidence of catalytic activity or protease inhibition, were designated non-peptidase homologs (NPH), and no reported protease inhibitory activity (NRPIA), respectively. MMP9 and TIMP1 were the most frequently published P/PIs and were reported in normal skin and most skin disease groups. Normal skin and diseased skin showed significant overlap with respect to P/PI profile; however, MMP23 was identified in several skin disease groups, but was absent in normal skin. The catalytic profile of P/PIs in wounds, scars and solar elastosis was distinct from normal skin, suggesting that a different group of P/PIs is responsible for disease progression. In conclusion, this study uses a novel approach to provide a comprehensive inventory of P/PIs in normal and diseased human skin reported in our database. The database may be used to determine either which P/PIs are present in specific diseases or which diseases individual P/PIs may influence. | |
dc.language | eng | |
dc.publisher | MDPI AG | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | nlmid: 101596414 | |
dc.source | essn: 2218-273X | |
dc.subject | Human | |
dc.subject | Protease | |
dc.subject | Proteome | |
dc.subject | Protease inhibitor | |
dc.subject | SKin | |
dc.title | Defining the Protease and Protease Inhibitor (P/PI) Proteomes of Healthy and Diseased Human Skin by Modified Systematic Review. | |
dc.type | Article | |
dc.date.updated | 2022-04-25T01:03:02Z | |
prism.issueIdentifier | 3 | |
prism.publicationName | Biomolecules | |
prism.volume | 12 | |
dc.identifier.doi | 10.17863/CAM.83825 | |
dcterms.dateAccepted | 2022-03-18 | |
rioxxterms.versionofrecord | 10.3390/biom12030475 | |
rioxxterms.version | VoR | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.contributor.orcid | Stewart-McGuinness, Callum [0000-0002-1572-4402] | |
dc.contributor.orcid | Platt, Christopher I [0000-0002-0525-3221] | |
dc.contributor.orcid | Ozols, Matiss [0000-0001-5663-1053] | |
dc.contributor.orcid | Goh, Brian [0000-0003-2253-1331] | |
dc.contributor.orcid | Sherratt, Michael J [0000-0003-4759-6617] | |
dc.identifier.eissn | 2218-273X | |
cam.issuedOnline | 2022-03-20 |
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