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dc.contributor.authorvan den Ameele, Jelle
dc.contributor.authorKrautz, Robert
dc.contributor.authorCheetham, Seth W
dc.contributor.authorDonovan, Alex PA
dc.contributor.authorLlorà-Batlle, Oriol
dc.contributor.authorYakob, Rebecca
dc.contributor.authorBrand, Andrea H
dc.date.accessioned2022-04-25T15:00:39Z
dc.date.available2022-04-25T15:00:39Z
dc.date.issued2022-04-25
dc.date.submitted2020-11-10
dc.identifier.others41467-022-29834-z
dc.identifier.other29834
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/336421
dc.descriptionFunder: Royal Society; doi: https://doi.org/10.13039/501100000288
dc.descriptionFunder: Herchel Smith Fund
dc.description.abstractAbstract: The Notch signalling pathway is a master regulator of cell fate transitions in development and disease. In the brain, Notch promotes neural stem cell (NSC) proliferation, regulates neuronal migration and maturation and can act as an oncogene or tumour suppressor. How NOTCH and its transcription factor RBPJ activate distinct gene regulatory networks in closely related cell types in vivo remains to be determined. Here we use Targeted DamID (TaDa), requiring only thousands of cells, to identify NOTCH and RBPJ binding in NSCs and their progeny in the mouse embryonic cerebral cortex in vivo. We find that NOTCH and RBPJ associate with a broad network of NSC genes. Repression of NSC-specific Notch target genes in intermediate progenitors and neurons correlates with decreased chromatin accessibility, suggesting that chromatin compaction may contribute to restricting NOTCH-mediated transactivation.
dc.languageen
dc.publisherNature Publishing Group UK
dc.subjectArticle
dc.subject/631/136/142
dc.subject/631/136/368
dc.subject/45/100
dc.subject/45/22
dc.subject/45/23
dc.subject/64/60
dc.subject/14/19
dc.subject/13/51
dc.subjectarticle
dc.titleReduced chromatin accessibility correlates with resistance to Notch activation
dc.typeArticle
dc.date.updated2022-04-25T15:00:38Z
prism.issueIdentifier1
prism.publicationNameNature Communications
prism.volume13
dc.identifier.doi10.17863/CAM.83839
dcterms.dateAccepted2022-03-18
rioxxterms.versionofrecord10.1038/s41467-022-29834-z
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidvan den Ameele, Jelle [0000-0002-2744-0810]
dc.contributor.orcidKrautz, Robert [0000-0003-0457-1348]
dc.contributor.orcidCheetham, Seth W. [0000-0001-6428-3175]
dc.contributor.orcidLlorà-Batlle, Oriol [0000-0002-8424-9705]
dc.contributor.orcidYakob, Rebecca [0000-0003-4275-5519]
dc.contributor.orcidBrand, Andrea H. [0000-0002-2089-6954]
dc.identifier.eissn2041-1723
pubs.funder-project-idWellcome Trust (Wellcome) (103792, 105839)


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