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dc.contributor.authorPruteanu, Lavinia-Lorena
dc.contributor.authorBraicu, Cornelia
dc.contributor.authorMódos, Dezső
dc.contributor.authorJurj, Maria-Ancuţa
dc.contributor.authorRaduly, Lajos-Zsolt
dc.contributor.authorZănoagă, Oana
dc.contributor.authorMagdo, Lorand
dc.contributor.authorCojocneanu, Roxana
dc.contributor.authorPaşca, Sergiu
dc.contributor.authorMoldovan, Cristian
dc.contributor.authorMoldovan, Alin Iulian
dc.contributor.authorŢigu, Adrian Bogdan
dc.contributor.authorGurzău, Eugen
dc.contributor.authorJäntschi, Lorentz
dc.contributor.authorBender, Andreas
dc.contributor.authorBerindan-Neagoe, Ioana
dc.date.accessioned2022-04-28T02:30:31Z
dc.date.available2022-04-28T02:30:31Z
dc.date.issued2022-04-26
dc.identifier.issn1661-6596
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/336537
dc.description.abstractTriple negative breast cancer (TNBC) is currently associated with a lack of treatment options. Arsenic derivatives have shown antitumoral activity both in vitro and in vivo; however, their mode of action is not completely understood. In this work we evaluate the response to arsenate of the double positive MCF-7 breast cancer cell line as well as of two different TNBC cell lines, Hs578T and MDA-MB-231. Multimodal experiments were conducted to this end, using functional assays and microarrays. Arsenate was found to induce cytoskeletal alteration, autophagy and apoptosis in TNBC cells, and moderate effects in MCF-7 cells. Gene expression analysis showed that the TNBC cell lines' response to arsenate was more prominent in the G2M checkpoint, autophagy and apoptosis compared to the Human Mammary Epithelial Cells (HMEC) and MCF-7 cell lines. We confirmed the downregulation of anti-apoptotic genes (MCL1, BCL2, TGFβ1 and CCND1) by qRT-PCR, and on the protein level, for TGFβ2, by ELISA. Insight into the mode of action of arsenate in TNBC cell lines it is provided, and we concluded that TNBC and non-TNBC cell lines reacted differently to arsenate treatment in this particular experimental setup. We suggest the future research of arsenate as a treatment strategy against TNBC.
dc.languageen
dc.publisherMDPI
dc.subjecttriple negative breast cancer
dc.subjectbreast cancer
dc.subjectgene expression
dc.subjectarsenate
dc.subjectmicroarray
dc.subjectmode of action
dc.titleTargeting Cell Death Mechanism Specifically in Triple Negative Breast Cancer Cell Lines.
dc.typeArticle
dc.date.updated2022-04-28T02:30:30Z
prism.issueIdentifier9
prism.publicationNameInt J Mol Sci
prism.volume23
dc.identifier.doi10.17863/CAM.83957
dcterms.dateAccepted2022-04-15
rioxxterms.versionofrecord10.3390/ijms23094784
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidBraicu, Cornelia [0000-0002-3055-4747]
dc.contributor.orcidMódos, Dezső [0000-0001-9412-6867]
dc.contributor.orcidRaduly, Lajos-Zsolt [0000-0002-3926-5423]
dc.contributor.orcidCojocneanu, Roxana [0000-0002-7450-9454]
dc.contributor.orcidMoldovan, Cristian [0000-0003-2927-4622]
dc.contributor.orcidMoldovan, Alin Iulian [0000-0001-7159-6102]
dc.contributor.orcidŢigu, Adrian Bogdan [0000-0001-9397-0791]
dc.contributor.orcidJäntschi, Lorentz [0000-0001-8524-743X]
dc.contributor.orcidBerindan-Neagoe, Ioana [0000-0001-5828-1325]
dc.identifier.eissn1422-0067
pubs.funder-project-idUnitatea Executiva Pentru Finantarea Invatamantului Superior a Cercetarii Dezvoltarii si Inovarii (PN-III-P1-1.2-PCCDI-2017-0737, CANCERTER-p53)


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