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dc.contributor.authorVan Den Ameele, Jelle
dc.contributor.authorKrautz, Robert
dc.contributor.authorCheetham, Seth W
dc.contributor.authorDonovan, Alex PA
dc.contributor.authorLlorà-Batlle, Oriol
dc.contributor.authorYakob, Rebecca
dc.contributor.authorBrand, Andrea
dc.description.abstractThe Notch signalling pathway is a master regulator of cell fate transitions in development and disease. In the brain, Notch promotes neural stem cell (NSC) proliferation, regulates neuronal migration and maturation and can act as an oncogene or tumour suppressor. How NOTCH and its transcription factor RBPJ activate distinct gene regulatory networks in closely related cell types in vivo remains to be determined. Here we use Targeted DamID (TaDa), requiring only thousands of cells, to identify NOTCH and RBPJ binding in NSCs and their progeny in the mouse embryonic cerebral cortex in vivo. We find that NOTCH and RBPJ associate with a broad network of NSC genes. Repression of NSC-specific Notch target genes in intermediate progenitors and neurons correlates with decreased chromatin accessibility, suggesting that chromatin compaction may contribute to restricting NOTCH-mediated transactivation.
dc.description.sponsorshipRoyal Society Darwin Trust Research Professorship; Herchel Smith Research Studentship
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International
dc.titleReduced chromatin accessibility correlates with resistance to Notch activation.
dc.publisher.departmentDepartment of Physiology, Development And Neuroscience
prism.publicationNameNat Commun
dc.contributor.orcidVan Den Ameele, Jelle [0000-0002-2744-0810]
dc.contributor.orcidKrautz, Robert [0000-0003-0457-1348]
dc.contributor.orcidCheetham, Seth W [0000-0001-6428-3175]
dc.contributor.orcidLlorà-Batlle, Oriol [0000-0002-8424-9705]
dc.contributor.orcidYakob, Rebecca [0000-0003-4275-5519]
dc.contributor.orcidBrand, Andrea [0000-0002-2089-6954]
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (103792/Z/14/Z)
pubs.funder-project-idWellcome Trust (105839/Z/14/Z)
pubs.funder-project-idRoyal Society (RP150061)
pubs.funder-project-idCancer Research UK (C6946/A24843)
pubs.funder-project-idWellcome Trust (203144/Z/16/Z)
pubs.licence-display-nameApollo Repository Deposit Licence Agreement

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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International