Genetic variation of macronutrient tolerance in Drosophila melanogaster.
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Authors
Ghazanfar, S
Hasygar, K
Pulpitel, T
Clark, T
Niranjan, HN
Shang, V
Tong, V
Publication Date
2022-03-28Journal Title
Nat Commun
ISSN
2041-1723
Publisher
Springer Science and Business Media LLC
Volume
13
Issue
1
Language
eng
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Havula, E., Ghazanfar, S., Lamichane, N., Francis, D., Hasygar, K., Liu, Y., Alton, L., et al. (2022). Genetic variation of macronutrient tolerance in Drosophila melanogaster.. Nat Commun, 13 (1) https://doi.org/10.1038/s41467-022-29183-x
Abstract
Carbohydrates, proteins and lipids are essential nutrients to all animals; however, closely related species, populations, and individuals can display dramatic variation in diet. Here we explore the variation in macronutrient tolerance in Drosophila melanogaster using the Drosophila genetic reference panel, a collection of ~200 strains derived from a single natural population. Our study demonstrates that D. melanogaster, often considered a "dietary generalist", displays marked genetic variation in survival on different diets, notably on high-sugar diet. Our genetic analysis and functional validation identify several regulators of macronutrient tolerance, including CG10960/GLUT8, Pkn and Eip75B. We also demonstrate a role for the JNK pathway in sugar tolerance and de novo lipogenesis. Finally, we report a role for tailless, a conserved orphan nuclear hormone receptor, in regulating sugar metabolism via insulin-like peptide secretion and sugar-responsive CCHamide-2 expression. Our study provides support for the use of nutrigenomics in the development of personalized nutrition.
Keywords
Animals, Drosophila, Drosophila melanogaster, DNA-Binding Proteins, Drosophila Proteins, Transcription Factors, Genetic Variation, Sugars, Nutrients
Identifiers
35347148, PMC8960806
External DOI: https://doi.org/10.1038/s41467-022-29183-x
This record's URL: https://www.repository.cam.ac.uk/handle/1810/336640
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