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dc.contributor.authorSirinakis, George
dc.contributor.authorAllgeyer, Edward S
dc.contributor.authorCheng, Jinmei
dc.contributor.authorSt Johnston, Daniel
dc.date.accessioned2022-05-09T23:30:52Z
dc.date.available2022-05-09T23:30:52Z
dc.date.issued2022-07-01
dc.identifier.issn2156-7085
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/336955
dc.description.abstractPAINT methods that use DNA- or protein- based exchangeable probes have become popular for super-resolution imaging and have been combined with spinning disk confocal microscopy for imaging thicker samples. However, the widely available spinning disks used for routine biological imaging are not optimized for PAINT-based applications and may compromise resolution and imaging speed. Here, we use Drosophila egg chambers in the presence of the actin-binding peptide Lifeact to study the performance of four different spinning disk geometries. We find that disk geometries with higher light collection efficiency perform better for PAINT-based super-resolution imaging due to increased photon numbers and, subsequently, detection of more blinking events.
dc.publisherOptica Publishing Group
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleQuantitative comparison of spinning disk geometries for PAINT based super-resolution microscopy.
dc.typeArticle
dc.publisher.departmentWellcome Trust/Cancer Research Uk Gurdon Institute
dc.date.updated2022-05-09T10:46:08Z
prism.publicationNameBiomed Opt Express
dc.identifier.doi10.17863/CAM.84378
dcterms.dateAccepted2022-05-02
rioxxterms.versionofrecord10.1364/BOE.459490
rioxxterms.versionAM
dc.contributor.orcidSirinakis, George [0000-0002-4762-422X]
dc.contributor.orcidAllgeyer, Edward S [0000-0002-2187-4423]
dc.identifier.eissn2156-7085
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (095927/B/11/Z)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/P026486/1)
pubs.funder-project-idWellcome Trust (203285/Z/16/Z)
pubs.funder-project-idWellcome Trust (092096/Z/10/Z)
pubs.funder-project-idWellcome Trust (203144/Z/16/Z)
pubs.funder-project-idWellcome Trust (080007/Z/06/Z)
pubs.funder-project-idWellcome Trust (080007/B/06/Z)
pubs.funder-project-idWellcome Trust (207496/Z/17/Z)
pubs.funder-project-idWellcome Trust (207496/Z/17/A)
pubs.funder-project-idCancer Research UK (C6946/A24843)
pubs.funder-project-idCancer Research Uk (None)
cam.issuedOnline2022-06-07
cam.depositDate2022-05-09
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International