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dc.contributor.authorHashmi, Ali
dc.contributor.authorTlili, Sham
dc.contributor.authorPerrin, Pierre
dc.contributor.authorLowndes, Molly
dc.contributor.authorPeradziryi, Hanna
dc.contributor.authorBrickman, Joshua M
dc.contributor.authorMartínez Arias, Alfonso
dc.contributor.authorLenne, Pierre-François
dc.date.accessioned2022-05-12T02:30:27Z
dc.date.available2022-05-12T02:30:27Z
dc.date.issued2022-04-11
dc.identifier.issn2050-084X
dc.identifier.other35404233
dc.identifier.otherPMC9033300
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/337059
dc.description.abstractShaping the animal body plan is a complex process that involves the spatial organization and patterning of the different germ layers. Recent advances in live imaging have started to unravel the cellular choreography underlying this process in mammals, however, the sequence of events transforming an unpatterned cell ensemble into structured territories is largely unknown. Here, using gastruloids -3D aggregates of mouse embryonic stem cells- we study the formation of one of the three germ layers, the endoderm. We show that the endoderm is generated from an epiblast-like homogeneous state by a three-step mechanism: (i) a loss of E-cadherin mediated contacts in parts of the aggregate leading to the appearance of islands of E-cadherin expressing cells surrounded by cells devoid of E-cadherin, (ii) a separation of these two populations with islands of E-cadherin expressing cells flowing toward the aggregate tip, and (iii) their differentiation into an endoderm population. During the flow, the islands of E-cadherin expressing cells are surrounded by cells expressing T-Brachyury, reminiscent of the process occurring at the primitive streak. Consistent with recent in vivo observations, the endoderm formation in the gastruloids does not require an epithelial-to-mesenchymal transition, but rather a maintenance of an epithelial state for a subset of cells coupled with fragmentation of E-cadherin contacts in the vicinity, and a sorting process. Our data emphasize the role of signaling and tissue flows in the establishment of the body plan.
dc.languageeng
dc.publishereLife Sciences Publications, Ltd
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcenlmid: 101579614
dc.sourceessn: 2050-084X
dc.subjectGerm Layers
dc.subjectEndoderm
dc.subjectAnimals
dc.subjectMammals
dc.subjectMice
dc.subjectCadherins
dc.subjectCell Differentiation
dc.subjectCell Movement
dc.subjectGastrulation
dc.titleCell-state transitions and collective cell movement generate an endoderm-like region in gastruloids.
dc.typeArticle
dc.date.updated2022-05-12T02:30:26Z
prism.publicationNameElife
prism.volume11
dc.identifier.doi10.17863/CAM.84482
dcterms.dateAccepted2022-04-08
rioxxterms.versionofrecord10.7554/eLife.59371
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidHashmi, Ali [0000-0001-9946-7679]
dc.contributor.orcidTlili, Sham [0000-0001-6018-9923]
dc.contributor.orcidBrickman, Joshua M [0000-0003-1580-7491]
dc.contributor.orcidMartínez Arias, Alfonso [0000-0002-1781-564X]
dc.contributor.orcidLenne, Pierre-François [0000-0003-1066-7506]
dc.identifier.eissn2050-084X
pubs.funder-project-idEuropean Research Council (834580)
cam.issuedOnline2022-04-11


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International