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dc.contributor.authorElías-Villalobos, Alberto
dc.contributor.authorDuncan, Caia
dc.contributor.authorMata, Juan
dc.contributor.authorHelmlinger, Dominique
dc.date.accessioned2022-05-12T23:30:26Z
dc.date.available2022-05-12T23:30:26Z
dc.date.issued2022-06
dc.identifier.issn2666-1667
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/337102
dc.description.abstractCharacterizing the interactions between RNAs and proteins in vivo is key to better understand how organisms regulate gene expression. Here, we describe a robust and quantitative protocol to measure specific RNA-protein interactions in a native context using RNA immunoprecipitation (RIP). We provide a comprehensive experimental framework to detect cotranslational interactions and detail the quantitative analysis of purified RNAs by PCR and high-throughput sequencing. Although we developed the protocol in fission yeast, it can be readily implemented in other yeast species.
dc.publisherElsevier BV
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleQuantitative analysis of protein-RNA interactions in fission yeast
dc.typeArticle
dc.publisher.departmentDepartment of Biochemistry
dc.date.updated2022-05-11T14:22:07Z
prism.endingPage101373
prism.issueIdentifier2
prism.number101373
prism.publicationDate2022
prism.publicationNameSTAR Protocols
prism.startingPage101373
prism.volume3
dc.identifier.doi10.17863/CAM.84521
dcterms.dateAccepted2022-05-09
rioxxterms.versionofrecord10.1016/j.xpro.2022.101373
rioxxterms.versionVoR
dc.contributor.orcidMata, Juan [0000-0002-5514-3653]
dc.identifier.eissn2666-1667
rioxxterms.typeJournal Article/Review
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/G011869/1)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/S015833/1)
cam.issuedOnline2022-05-09
cam.depositDate2022-05-11
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement


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Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial-NoDerivatives 4.0 International