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dc.contributor.authorAnang, Dornatien Chuo
dc.contributor.authorRamwadhdoebe, Tamara H
dc.contributor.authorHähnlein, Janine S
dc.contributor.authorvan Kuijk, Bo
dc.contributor.authorSmits, Noortje
dc.contributor.authorvan Lienden, Krijn P
dc.contributor.authorMaas, Mario
dc.contributor.authorGerlag, Daniëlle M
dc.contributor.authorTak, Paul P
dc.contributor.authorde Vries, Niek
dc.contributor.authorvan Baarsen, Lisa GM
dc.date.accessioned2022-05-14T01:06:26Z
dc.date.available2022-05-14T01:06:26Z
dc.date.issued2022-03-24
dc.identifier.issn2073-4409
dc.identifier.other35406668
dc.identifier.otherPMC8997933
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/337160
dc.description.abstractFollicular T helper cells (Tfh cells) provide key B-cell help and are essential in germinal center formation and (auto) antibody generation. To gain more insight into their role during the earliest phase of rheumatoid arthritis (RA), we analyzed their frequencies, phenotypes, and cytokine profiles in peripheral blood and lymph node biopsies of healthy controls (HCs), autoantibody-positive individuals at risk for developing RA (RA-risk individuals), and early RA patients. Subsequently, we confirmed their presence in lymph nodes and synovial tissue of RA patients using immunofluorescence microscopy. In the blood, the frequency of Tfh cells did not differ between study groups. In lymphoid and synovial tissues, Tfh cells were localized in B-cell areas, and their frequency correlated with the frequency of CD19+ B cells. Compared to lymphoid tissues of healthy controls, those of RA patients and RA-risk individuals showed more CD19+ B cells, CD4+CXCR5+ follicular helper T cells, and CD8+CXCR5+ follicular T cells. These Tfh cells produced less IL-21 upon ex vivo stimulation. These findings suggest that Tfh cells may present a novel rationale for therapeutic targeting during the preclinical stage of RA to prevent further disease progression.
dc.languageeng
dc.publisherMDPI AG
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcenlmid: 101600052
dc.sourceessn: 2073-4409
dc.subjectLymphoid Tissue
dc.subjectLymph Nodes
dc.subjectT-Lymphocytes, Helper-Inducer
dc.subjectCD8-Positive T-Lymphocytes
dc.subjectHumans
dc.subjectArthritis, Rheumatoid
dc.subjectBiopsy
dc.titleIncreased Frequency of CD4+ Follicular Helper T and CD8+ Follicular T Cells in Human Lymph Node Biopsies during the Earliest Stages of Rheumatoid Arthritis.
dc.typeArticle
dc.date.updated2022-05-14T01:06:25Z
prism.issueIdentifier7
prism.publicationNameCells
prism.volume11
dc.identifier.doi10.17863/CAM.84579
dcterms.dateAccepted2022-03-22
rioxxterms.versionofrecord10.3390/cells11071104
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidAnang, Dornatien Chuo [0000-0001-5454-2639]
dc.contributor.orcidTak, Paul P [0000-0002-3532-5409]
dc.contributor.orcidvan Baarsen, Lisa GM [0000-0003-3346-8921]
dc.identifier.eissn2073-4409
cam.issuedOnline2022-03-24


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International