Show simple item record

dc.contributor.authorKorona, Dagmara
dc.contributor.authorDirnberger, Benedict
dc.contributor.authorGiachello, Carlo NG
dc.contributor.authorQueiroz, Rayner ML
dc.contributor.authorPopovic, Rebeka
dc.contributor.authorMüller, Karin H
dc.contributor.authorMinde, David-Paul
dc.contributor.authorDeery, Michael J
dc.contributor.authorJohnson, Glynnis
dc.contributor.authorFirth, Lucy C
dc.contributor.authorEarley, Fergus G
dc.contributor.authorRussell, Steven
dc.contributor.authorLilley, Kathryn S
dc.date.accessioned2022-05-17T09:03:48Z
dc.date.available2022-05-17T09:03:48Z
dc.date.issued2022-05-16
dc.date.submitted2021-09-29
dc.identifier.other74322
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/337228
dc.description.abstract<jats:p><jats:italic>Drosophila</jats:italic> nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels that represent a target for insecticides. Peptide neurotoxins are known to block nAChRs by binding to their target subunits, however, a better understanding of this mechanism is needed for effective insecticide design. To facilitate the analysis of nAChRs we used a CRISPR/Cas9 strategy to generate null alleles for all ten <jats:italic>nAChR</jats:italic> subunit genes in a common genetic background. We studied interactions of nAChR subunits with peptide neurotoxins by larval injections and styrene maleic acid lipid particles (SMALPs) pull-down assays. For the null alleles, we determined the effects of α-Bungarotoxin (α-Btx) and ω-Hexatoxin-Hv1a (Hv1a) administration, identifying potential receptor subunits implicated in the binding of these toxins. We employed pull-down assays to confirm α-Btx interactions with the <jats:italic>Drosophila</jats:italic> α5 (D<jats:italic>α</jats:italic>5), Dα6, D<jats:italic>α</jats:italic>7 subunits. Finally, we report the localisation of fluorescent tagged endogenous Dα6 during <jats:italic>Drosophila</jats:italic> CNS development. Taken together, this study elucidates native <jats:italic>Drosophila</jats:italic> nAChR subunit interactions with insecticidal peptide toxins and provides a resource for the in vivo analysis of insect nAChRs.</jats:p>
dc.languageen
dc.publishereLife Sciences Publications, Ltd
dc.subjectResearch Article
dc.subjectNeuroscience
dc.subjectDrosophila nAChRs
dc.subjectneurotoxin interactions
dc.subjectinsecticidal toxins
dc.subjectDrosophila
dc.subjectD. melanogaster
dc.titleDrosophila nicotinic acetylcholine receptor subunits and their native interactions with insecticidal peptide toxins
dc.typeArticle
dc.date.updated2022-05-17T09:03:46Z
prism.publicationNameeLife
prism.volume11
dc.identifier.doi10.17863/CAM.84646
dcterms.dateAccepted2022-04-19
rioxxterms.versionofrecord10.7554/elife.74322
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
datacite.contributor.supervisoreditor: Pless, Stephan A
datacite.contributor.supervisorsenior_editor: VijayRaghavan, K
dc.contributor.orcidKorona, Dagmara [0000-0002-5988-3894]
dc.contributor.orcidDirnberger, Benedict [0000-0002-0772-5923]
dc.contributor.orcidEarley, Fergus G [0000-0003-1943-0724]
dc.identifier.eissn2050-084X
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/P021107/1)
cam.issuedOnline2022-05-16


Files in this item

Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record