Interspecific hybridization in tomato influences endogenous viral sRNAs and alters gene expression.

Lopez-Gomollon, Sara  ORCID logo
Müller, Sebastian Y  ORCID logo

Thumbnail Image
Change log

BACKGROUND: Hybridization is associated with the activation of transposable elements and changes in the patterns of gene expression leading to phenotypic changes. However, the underlying mechanisms are not well understood. RESULTS: Here, we describe the changes to the gene expression in interspecific Solanum hybrids that are associated with small RNAs derived from endogenous (para)retroviruses (EPRV). There were prominent changes to sRNA profiles in these hybrids involving 22-nt species produced in the DCL2 biogenesis pathway, and the hybridization-induced changes to the gene expression were similar to those in a dcl2 mutant. CONCLUSIONS: These findings indicate that hybridization leads to activation of EPRV, perturbation of small RNA profiles, and, consequently, changes in the gene expression. Such hybridization-induced variation in the gene expression could increase the natural phenotypic variation in natural evolution or in breeding for agriculture.

Publication Date
Online Publication Date
Acceptance Date
Changes in gene expression, DCL2 (Dicer-like 2), Endogenous pararetroviruses, Hybridization, RNA silencing, Small RNAs, Transposable elements, DNA Transposable Elements, Gene Expression, Hybridization, Genetic, Solanum lycopersicum, Plant Breeding, RNA
Journal Title
Genome Biol
Journal ISSN
Volume Title
Springer Science and Business Media LLC
Royal Society (RP120015)
European Research Council (233325)
European Research Council (340642)
Royal Society (RP170001)
This work was supported by the European Research Council Advanced Investigator grant ERC-2013-AdG 340642 (Transgressive Inheritance in Plant Breeding and Evolution [TRIBE]), the Royal Society (RP170001), Syngenta, the Biotechnology and Biological Sciences Research Council (RG68461), the Balzan Foundation, and the Broodbank Fund. SLG is a Senior Broodbank Research Fellow. D.C.B. is the Royal Society Edward Penley Abraham Research Professor.
Is supplemented by: