Loss of Function of the Neural Cell Adhesion Molecule NrCAM Regulates Differentiation, Proliferation and Neurogenesis in Early Postnatal Hypothalamic Tanycytes.
Kim, Dong Won
Dowsett, Georgina KC
Lewis, Jo E
Frontiers Media SA
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Moore, A., Chinnaiya, K., Kim, D. W., Brown, S., Stewart, I., Robins, S., Dowsett, G. K., et al. (2022). Loss of Function of the Neural Cell Adhesion Molecule NrCAM Regulates Differentiation, Proliferation and Neurogenesis in Early Postnatal Hypothalamic Tanycytes.. Front Neurosci, 16 https://doi.org/10.3389/fnins.2022.832961
Hypothalamic tanycytes are neural stem and progenitor cells, but little is known of how they are regulated. Here we provide evidence that the cell adhesion molecule, NrCAM, regulates tanycytes in the adult niche. NrCAM is strongly expressed in adult mouse tanycytes. Immunohistochemical and in situ hybridization analysis revealed that NrCAM loss of function leads to both a reduced number of tanycytes and reduced expression of tanycyte-specific cell markers, along with a small reduction in tyrosine hydroxylase-positive arcuate neurons. Similar analyses of NrCAM mutants at E16 identify few changes in gene expression or cell composition, indicating that NrCAM regulates tanycytes, rather than early embryonic hypothalamic development. Neurosphere and organotypic assays support the idea that NrCAM governs cellular homeostasis. Single-cell RNA sequencing (scRNA-Seq) shows that tanycyte-specific genes, including a number that are implicated in thyroid hormone metabolism, show reduced expression in the mutant mouse. However, the mild tanycyte depletion and loss of markers observed in NrCAM-deficient mice were associated with only a subtle metabolic phenotype.
NrCAM, astrocytes, hypothalamus, neural cell adhesion molecules, neurogenesis, radial glia, scRNA seq, tanycyte
Wellcome Trust (212247/Z/18/Z)
External DOI: https://doi.org/10.3389/fnins.2022.832961
This record's URL: https://www.repository.cam.ac.uk/handle/1810/337496
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/
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