Show simple item record

dc.contributor.authorBrand, Andrea
dc.contributor.authorVan Den Ameele, jelle
dc.contributor.authorKrautz, robert
dc.contributor.authorCheetham, Seth
dc.contributor.authorDonovan, Alex PA
dc.contributor.authorLlorà-Batlle, Oriol
dc.contributor.authorYakob, Rebecca
dc.date.accessioned2022-05-27T01:02:50Z
dc.date.available2022-05-27T01:02:50Z
dc.date.issued2022-04-25
dc.identifier.issn2041-1723
dc.identifier.other35468895
dc.identifier.otherPMC9039071
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/337526
dc.descriptionFunder: Royal Society
dc.descriptionFunder: Herchel Smith Fund
dc.description.abstractThe Notch signalling pathway is a master regulator of cell fate transitions in development and disease. In the brain, Notch promotes neural stem cell (NSC) proliferation, regulates neuronal migration and maturation and can act as an oncogene or tumour suppressor. How NOTCH and its transcription factor RBPJ activate distinct gene regulatory networks in closely related cell types in vivo remains to be determined. Here we use Targeted DamID (TaDa), requiring only thousands of cells, to identify NOTCH and RBPJ binding in NSCs and their progeny in the mouse embryonic cerebral cortex in vivo. We find that NOTCH and RBPJ associate with a broad network of NSC genes. Repression of NSC-specific Notch target genes in intermediate progenitors and neurons correlates with decreased chromatin accessibility, suggesting that chromatin compaction may contribute to restricting NOTCH-mediated transactivation.
dc.description.sponsorshipRoyal Society Darwin Trust Research Professorship; Herchel Smith Research Studentship
dc.languageeng
dc.publisherNature Research
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcenlmid: 101528555
dc.sourceessn: 2041-1723
dc.subjectChromatin
dc.subjectAnimals
dc.subjectMice
dc.subjectSignal Transduction
dc.subjectCell Differentiation
dc.subjectReceptors, Notch
dc.subjectNeural Stem Cells
dc.titleReduced chromatin accessibility correlates with resistance to Notch activation
dc.typeArticle
dc.date.updated2022-05-27T01:02:50Z
prism.issueIdentifier1
prism.publicationNameNature Communications
prism.volume13
dc.identifier.doi10.17863/CAM.84941
dcterms.dateAccepted2022-03-18
rioxxterms.versionofrecord10.1038/s41467-022-29834-z
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidvan den Ameele, Jelle [0000-0002-2744-0810]
dc.contributor.orcidKrautz, Robert [0000-0003-0457-1348]
dc.contributor.orcidCheetham, Seth W [0000-0001-6428-3175]
dc.contributor.orcidLlorà-Batlle, Oriol [0000-0002-8424-9705]
dc.contributor.orcidYakob, Rebecca [0000-0003-4275-5519]
dc.contributor.orcidBrand, Andrea H [0000-0002-2089-6954]
dc.identifier.eissn2041-1723
pubs.funder-project-idWellcome Trust (103792/Z/14/Z)
pubs.funder-project-idWellcome Trust (105839/Z/14/Z)
pubs.funder-project-idRoyal Society (RP150061)
pubs.funder-project-idCancer Research UK (C6946/A24843)
pubs.funder-project-idWellcome Trust (203144/Z/16/Z)
cam.issuedOnline2022-04-25


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International