Research data supporting "A root phloem pole cell atlas reveals common transcriptional states in protophloem adjacent cells"
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Description
The data contains confocal images, plate scans and construct maps. The materials are divided by figure number. The folders contains original confocal images (.lif), plasmid maps (.ape or .str, can be opened with free software A plasmid editor), scanned plates with seedlings to measure root growth (.tif or .jpg files) and excel/.csv files with measurements. ccc is 3papl, p1p2 is pear1pear2 mutant, se6 is pear sextuple mutant, suc means the media contains sucrose and 0suc means no sucrose in the media. dps means days post sowing. There is an Excel file ('Plasmids_Otero_etal copy') for the plasmids used in this manuscript. The ID column is an internal lab code.
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Software / Usage instructions
Confocal pictures can be opened either with Image J or Leica LAS X software
Csv files could be opened with Excel
Plasmid maps could be opened with A plasmid editor (ApE)
Scans could be opened with any image processor
Keywords
Arabidopsis root, Arabidopsis thaliana, companion cells, DOF transcription factors, metaphloem sieve element, PEAR genes, phloem, phloem pole pericycle, plant biology, protophloem sieve element, root development, root phloem poles, single cell RNAseq, vascular development