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dc.contributor.authorClark, Bethan
dc.contributor.authorElkin, Joel
dc.contributor.authorMarconi, Aleksandra
dc.contributor.authorTurner, George F.
dc.contributor.authorSmith, Alan M.
dc.contributor.authorJoyce, Domino
dc.contributor.authorMiska, Eric A.
dc.contributor.authorJuntti, Scott A.
dc.contributor.authorSantos, M. Emília
dc.date.accessioned2022-05-27T20:00:03Z
dc.date.available2022-05-27T20:00:03Z
dc.date.issued2022-04-20
dc.date.submitted2022-01-20
dc.identifier.otherrsos220077
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/337563
dc.description.abstractIdentifying genetic loci underlying trait variation provides insights into the mechanisms of diversification, but demonstrating causality and characterizing the role of genetic loci requires testing candidate gene function, often in non-model species. Here we establish CRISPR/Cas9 editing in Astatotilapia calliptera, a generalist cichlid of the remarkably diverse Lake Malawi radiation. By targeting the gene oca2 required for melanin synthesis in other vertebrate species, we show efficient editing and germline transmission. Gene edits include indels in the coding region, probably a result of non-homologous end joining, and a large deletion in the 3′ untranslated region due to homology-directed repair. We find that oca2 knock-out A. calliptera lack melanin, which may be useful for developmental imaging in embryos and studying colour pattern formation in adults. As A. calliptera resembles the presumed generalist ancestor of the Lake Malawi cichlid radiation, establishing genome editing in this species will facilitate investigating speciation, adaptation and trait diversification in this textbook radiation.
dc.languageen
dc.publisherThe Royal Society
dc.subjectOrganismal and evolutionary biology
dc.subjectResearch articles
dc.subjectcichlids
dc.subjectCRISPR/Cas9
dc.subjectadaptive radiation
dc.subjectfunctional genetics
dc.subjectevo-devo
dc.titleOca2 targeting using CRISPR/Cas9 in the Malawi cichlid Astatotilapia calliptera
dc.typeArticle
dc.date.updated2022-05-27T20:00:02Z
prism.issueIdentifier4
prism.publicationNameRoyal Society Open Science
prism.volume9
dc.identifier.doi10.17863/CAM.84972
dcterms.dateAccepted2022-03-22
rioxxterms.versionofrecord10.1098/rsos.220077
rioxxterms.versionAO
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidJoyce, Domino [0000-0002-4253-5623]
dc.contributor.orcidSantos, M. Emília [0000-0003-3158-7935]
dc.identifier.eissn2054-5703
pubs.funder-project-idCancer Research UK (C13474/A18583, C6946/A14492)
pubs.funder-project-idHuman Frontier Science Program (RGY0079/2018)
pubs.funder-project-idNSF (IOS-1825723)
pubs.funder-project-idNatural Environment Research Council (NE/R01504X/1)
pubs.funder-project-idWellcome Trust (092096/Z/10/Z, 102175/Z/13/Z, 219475/Z/19/Z, 222279/Z/20/Z)


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