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dc.contributor.authorKurapova, Regina
dc.contributor.authorChouliaras, Leonidas
dc.contributor.authorO'Brien, John T
dc.date.accessioned2022-06-01T23:30:58Z
dc.date.available2022-06-01T23:30:58Z
dc.date.issued2022-08
dc.identifier.issn0531-5565
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/337682
dc.description.abstractLewy body dementia encompasses the common neurodegenerative disorders Dementia with Lewy bodies (DLB) and Parkinson's disease dementia (PDD). Lewy Body disease (LBD) is characterized by abnormal aggregates of α-synuclein (α-syn) in the brain which form Lewy bodies. LBD is commonly misdiagnosed/underdiagnosed, especially in early stages. There remains a great need for reliable biomarkers to assist with LBD diagnosis. Amplification techniques such as real-time quaking-induced conversion (RT-QuIC) and protein misfolding cyclic amplification (PMCA) represent an important advance for biomarker detection. Amplification assays detect the ability of pathogenic protein to induce conformational change in normal protein; α-syn has been shown to propagate in a prion-like manner, making it a candidate for such analysis. In this review, we describe the diagnostic potential of amplification techniques for differentiating α-synucleinopathies from other neurodegenerative disorders such as Alzheimer's disease (AD), frontotemporal dementia (FTD), progressive supranuclear palsy (PSP), corticobasal syndrome (CBS), and atypical parkinsonism, as well as α-synucleinopathies from each other. Recent studies report accurate detection of α-syn seeding activity in human tissues such as cerebrospinal fluid (CSF), submandibular gland (SMG), and posterior cervical skin. Adaptation to clinical settings may present challenges. However, the high accuracy of recent results, combined with the success of amplification assay diagnostics in clinical practice for Creutzfeldt-Jakob disease, suggest high promise for eventual clinical application.
dc.publisherElsevier BV
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleThe promise of amplification assays for accurate early detection of α-synucleinopathies: A review.
dc.typeArticle
dc.publisher.departmentDepartment of Psychiatry
dc.date.updated2022-06-01T09:58:15Z
prism.endingPage111842
prism.number111842
prism.publicationDate2022
prism.publicationNameExp Gerontol
prism.startingPage111842
dc.identifier.doi10.17863/CAM.85088
dcterms.dateAccepted2022-05-20
rioxxterms.versionofrecord10.1016/j.exger.2022.111842
rioxxterms.versionAM
dc.contributor.orcidO'Brien, John [0000-0002-0837-5080]
dc.identifier.eissn1873-6815
rioxxterms.typeJournal Article/Review
cam.issuedOnline2022-05-24
cam.orpheus.success2022-06-01 - Embargo set during processing via Fast-track
cam.depositDate2022-06-01
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement
rioxxterms.freetoread.startdate2023-05-24


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Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial-NoDerivatives 4.0 International